The objectives of the proposed project are: 1. To use the technique of Energy Dispersive X-ray Spectroscopy (EDS) to quantify calcium and other biologically important elements in subcellular regions of mammalian myocardium known to bind or sequester calcium (terminal cisternae, mitochondria, I-band). 2. To measure changes in the concentrations of calcium and other elements at these sites under conditions which have been hypothesized to directly affect storage or release of calcium in myocardium. In particular, we will investigate the effects of a) caffeine, b) varying extracellular calcium, and c) varying sarcomere length on resting and tetanized (in the presence of caffeine) ventricular muscle. 3. To adapt a relatively new freezing method (propane jet freezing) for the study of intact myocardial tissue, with the ultimate goal of studying beat-to-beat variations in subcellular elemental distributions.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Unknown (R23)
Project #
5R23HL031444-03
Application #
3448605
Study Section
Cardiovascular Study Section (CVA)
Project Start
1984-01-01
Project End
1986-12-31
Budget Start
1986-01-01
Budget End
1986-12-31
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of Washington
Department
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
Cantino, M E; Wilkinson, L E; Goddard, M K et al. (1986) Beam induced mass loss in high resolution biological microanalysis. J Microsc 144:317-27