Flow cytometry technology is a powerful tool to study cancer cell biology. Using monoclonal antibodies, molecular probes and chemicals labelled with flurochromes with different excitation- emission spectra single and multiple cell surface markers, apoptosis, cell activation status, cell cycle kinetics, gene and gene product expression can be measured in individual cells. Moreover, this technology has the capacity to rapidly isolate cells that display common or different parameters within a mixed population.
The Aims of this proposal are to provide: 1) State-of-the-art instrumentation, b) professional oversite, and c) technical support for NCI supported investigators conducting a variety of studies in cancer cell biology. These funded studies include the following: Multi-drug resistance in mammalian cells and mechanism of drug resistance in acute myeloid leukemia, folate transport and antifolate resistance, regulation of the DNA synthesone in normal and malignant breast cells, characterization of the cytotoxic drug resistance phenotype of cells expressing the breast cancer resistance protein, mechanism of IL-10 activity in breast cancer therapy, investigation of ErbB receptor control of breast cancer cell growth, the role of IGFR (I and II) in GI malignancies, and the role of microsatellite instability in colorectal cancers. Flow cytometry technology will greatly enhance the ability of these investigators to achieve the goals and aims of their research efforts.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Resource-Related Research Projects (R24)
Project #
5R24CA082888-02
Application #
6174404
Study Section
Special Emphasis Panel (ZCA1-SRRB-C (M1))
Program Officer
Spalholz, Barbara A
Project Start
1999-08-01
Project End
2002-07-31
Budget Start
2000-08-01
Budget End
2001-07-31
Support Year
2
Fiscal Year
2000
Total Cost
$185,625
Indirect Cost
Name
University of Maryland Baltimore
Department
Pathology
Type
Schools of Medicine
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201
Stephenson, Andrew J; Scardino, Peter T; Eastham, James A et al. (2005) Postoperative nomogram predicting the 10-year probability of prostate cancer recurrence after radical prostatectomy. J Clin Oncol 23:7005-12
Klyushnenkova, Elena N; Ponniah, Sathibalan; Rodriguez, Alejandro et al. (2004) CD4 and CD8 T-lymphocyte recognition of prostate specific antigen in granulomatous prostatitis. J Immunother 27:136-46
Rice, Anna M; Holtz, Kathleen M; Karp, Judith et al. (2004) Analysis of the relationship between Scl transcription factor complex protein expression patterns and the effects of LiCl on ATRA-induced differentiation in blast cells from patients with acute myeloid leukemia. Leuk Res 28:1227-37
Karp, Judith E; Ross, Douglas D; Yang, Weidong et al. (2003) Timed sequential therapy of acute leukemia with flavopiridol: in vitro model for a phase I clinical trial. Clin Cancer Res 9:307-15
Kundu, Namita; Smyth, Miriam J; Samsel, Leigh et al. (2002) Cyclooxygenase inhibitors block cell growth, increase ceramide and inhibit cell cycle. Breast Cancer Res Treat 76:57-64
Berlyn, K A; Schultes, B; Leveugle, B et al. (2001) Generation of CD4(+) and CD8(+) T lymphocyte responses by dendritic cells armed with PSA/anti-PSA (antigen/antibody) complexes. Clin Immunol 101:276-83
Rapoport, A P; Simons-Evelyn, M; Chen, T et al. (2001) Flavopiridol induces apoptosis and caspase-3 activation of a newly characterized Burkitt's lymphoma cell line containing mutant p53 genes. Blood Cells Mol Dis 27:610-24