Creation of a Small Animal Imaging Resource Program (SAIRP) at the University of Pennsylvania supporting research by investigators at this institution and also at the Fox Chase Cancer Center and Thomas Jefferson University is proposed. This SAIRP will provide three imaging modalities -- 1) Magnetic Resonance Imaging and Spectroscopy (MRI/S). 2) optical imaging covering the ultraviolet through near infrared (UV-NIR), and positron emission tomography (PET). The MRI/S facilities are now in place; the UV-NIR and PET facilities are now operational on the scale of human patients and will be adapted to studies of small animals (mice and rats) by the end of the first year. The SAIRP will support the research of 13 NIH funded projects dealing with 1) modification of tumor response to radiation and hyperthermia, 2) methods of monitoring tumor hypoxia, 3) gene therapy of brain tumors, 4) immunotherapy of tumors, 5) detection of breast cancer and 6) detection of tumor response to chemotherapy and radiation therapy. Ancillary facilities for redox scanning, NIR time resolved spectroscopoy, electronics, animal management, synthesis of contrast agent and physiological probes, histology, computer resources, biostatistics and MR of perfused cells will be provided. Research and development and D) projects will be directed towards the development of novel NMR capabilities that will enhance the research capabilities of the base projects. These R and D projects are: A. MRI of Small Animal Tumors, including: A1. Small Animal Microimaging. A2. Functional Imaging of Tumors, B. Multinuclear Spectroscopy, including: B1. Chemical Shift Selective MR Imaging of 31P in Animal Tumors, B2. 1H{17O} MRI of Tumors, B3. In Vivo Imaging of Intra- and Extracellular Na+ and pH in Tumors, and C. NMR Techniques for Monitoring Gene Therapy of Brain Tumors.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Resource-Related Research Projects (R24)
Project #
5R24CA083105-04
Application #
6522326
Study Section
Special Emphasis Panel (ZCA1-SRRB-3 (M2))
Program Officer
Croft, Barbara
Project Start
1999-09-01
Project End
2004-07-31
Budget Start
2002-08-01
Budget End
2003-07-31
Support Year
4
Fiscal Year
2002
Total Cost
$562,734
Indirect Cost
Name
University of Pennsylvania
Department
Radiation-Diagnostic/Oncology
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Cai, Kejia; Xu, He N; Singh, Anup et al. (2013) Characterizing prostate tumor mouse xenografts with CEST and MT-MRI and redox scanning. Adv Exp Med Biol 765:39-45
Mawn, Theresa M; Popov, Anatoliy V; Delikatny, E James (2012) A quantitative continuous enzyme assay of intramolecularly quenched fluorogenic phospholipase substrates for molecular imaging. Anal Biochem 422:96-102
Mawn, Theresa M; Popov, Anatoliy V; Beardsley, Nancy J et al. (2011) In vivo detection of phospholipase C by enzyme-activated near-infrared probes. Bioconjug Chem 22:2434-43
Milkevitch, Matthew; Beardsley, Nancy J; Delikatny, E James (2010) Phenylbutyrate induces apoptosis and lipid accumulations via a peroxisome proliferator-activated receptor gamma-dependent pathway. NMR Biomed 23:473-9
Mellon, Eric A; Lee, Seung-Cheol; Pickup, Stephen et al. (2009) Detection of lactate with a hadamard slice selected, selective multiple quantum coherence, chemical shift imaging sequence (HDMD-SelMQC-CSI) on a clinical MRI scanner: Application to tumors and muscle ischemia. Magn Reson Med 62:1404-13
Glickson, Jerry D; Lund-Katz, Sissel; Zhou, Rong et al. (2009) Lipoprotein nanoplatform for targeted delivery of diagnostic and therapeutic agents. Adv Exp Med Biol 645:227-39
Glickson, Jerry D; Lund-Katz, Sissel; Zhou, Rong et al. (2008) Lipoprotein nanoplatform for targeted delivery of diagnostic and therapeutic agents. Mol Imaging 7:101-10
Milkevitch, Matthew; Jeitner, Thomas M; Beardsley, Nancy J et al. (2007) Lovastatin enhances phenylbutyrate-induced MR-visible glycerophosphocholine but not apoptosis in DU145 prostate cells. Biochim Biophys Acta 1771:1166-76
Zheng, Gang; Chen, Juan; Li, Hui et al. (2005) Rerouting lipoprotein nanoparticles to selected alternate receptors for the targeted delivery of cancer diagnostic and therapeutic agents. Proc Natl Acad Sci U S A 102:17757-62
Li, Zijun; Qiao, Hui; Lebherz, Corinna et al. (2005) Creatine kinase, a magnetic resonance-detectable marker gene for quantification of liver-directed gene transfer. Hum Gene Ther 16:1429-38

Showing the most recent 10 out of 32 publications