Studies of normal and malignant human hematopoietic cells (including immune cells (T, B, monocytes), bone marrow progenitors and stem cells) encompass the entire spectrum of cancer research, from basic mechanisms to new diagnostic and therapeutic approaches. However, generating sufficient numbers of specific primary human cells for such studies often requires leukopheresis capability (e.g. to obtain large numbers of normal donor peripheral blood mononuclear cells for purification of T cells), access to patient samples, large scale purification methodology (necessitating large scale antibody production for immunomagnetic approaches), quality control, cell freezers, inventory and database infrastructure (especially for patient samples) that are beyond the resources of individual laboratories. This """"""""functional"""""""" inaccessibility to primary human hematopoietic and immune cells could be solved by a Shared Resource that efficiently and economically provides primary human cells to a wide group of researchers, a resource that does not currently exist at the University of Miami. Such a resource would significantly enhance and expand ongoing cancer research, as well as research in human immunology, hematology and infectious disease. The goal of this proposal is to establish the Cell Purification and Banking Facility (CPBF) to provide viable, purified primary human hematopoietic cells (normal T cells, B cells, monocytes, CD34 stem cells and primary leukemia, lymphoma and myeloma isolates) to cancer researchers at the University of Miami. To accomplish this, the CPBF will integrate recently established infrastructure components (Research Apheresis Unit, Cell Purification Unit (including antibody production and quality control), Cell Bank and an inventory/sample database (with a web-based search and ordering interface) into a single Shared Resource. In addition, the CPBF will provide banking, inventory and database services for cancer-related clinical trials that are collecting cell samples for research.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Resource-Related Research Projects (R24)
Project #
5R24CA095829-04
Application #
6881594
Study Section
Special Emphasis Panel (ZCA1-SRRB-Y (J2))
Program Officer
Mufson, R Allan
Project Start
2002-04-01
Project End
2007-03-31
Budget Start
2005-04-01
Budget End
2006-03-31
Support Year
4
Fiscal Year
2005
Total Cost
$160,743
Indirect Cost
Name
University of Miami School of Medicine
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
052780918
City
Miami
State
FL
Country
United States
Zip Code
33146
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Bahlis, Nizar J; King, Anne M; Kolonias, Despina et al. (2007) CD28-mediated regulation of multiple myeloma cell proliferation and survival. Blood 109:5002-10
Cejas, Pedro J; Carlson, Louise M; Zhang, Jian et al. (2005) Protein kinase C betaII plays an essential role in dendritic cell differentiation and autoregulates its own expression. J Biol Chem 280:28412-23
Kharfan-Dabaja, Mohamed; Ayala, Ernesto; Lindner, Inna et al. (2005) Differentiation of acute and chronic myeloid leukemic blasts into the dendritic cell lineage: analysis of various differentiation-inducing signals. Cancer Immunol Immunother 54:25-36
Lindner, Inna; Kharfan-Dabaja, Mohamed A; Ayala, Ernesto et al. (2003) Induced dendritic cell differentiation of chronic myeloid leukemia blasts is associated with down-regulation of BCR-ABL. J Immunol 171:1780-91