Abstract

BresaGen, Inc has generated four human embryonic stem cell (hESC) lines that can be used for National Institutes of Health (NIH) funded research projects. To make this valuable resource available to the research community, BresaGen has licensed these cells to Cellsaurus, Inc. BresaGen will also provide technical support to Cellsaurus to enable the efficient transfer of hESCs and technologies. Cellsaurus will produce and distribute the hESC lines, and will develop and document methods and standard operating procedures that will be made available to the customers. The work proposed in this application has three Specific Aims.
In Aim 1 : hESCs will be produced and distributed using the methods, protocols and quality control criteria already developed by BresaGen. Cell production and distribution will adhere to GLP specifications. The experiments of Aim 2: will develop novel methods for the efficient expansion of the cells and will develop new markers to be used in hESC quality control.
Aim 3 : is focused on the development of mechanisms to ensure the success of Cellsaurus customers.
This Aim i ncludes the development of an efficient hESC distribution system, the creation of a technical manual and a technical support website, and the establishment of a hands-on training workshop. Cellsaurus will become an independent entity through recovery of costs from customers, arid by the royalty stream associated with the capture of intellectual property rights.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Resource-Related Research Projects (R24)
Project #
5R24DK063689-03
Application #
6612569
Study Section
Special Emphasis Panel (ZRR1-CR-A (01))
Program Officer
Hammond, Robert D
Project Start
2002-05-01
Project End
2004-09-16
Budget Start
2003-05-01
Budget End
2004-09-16
Support Year
3
Fiscal Year
2003
Total Cost
$458,890
Indirect Cost

  Institution

Name
Bresagen, Inc.
Department
Type
DUNS #
009468930
City
Athens
State
GA
Country
United States
Zip Code
30602

  Publications

Zeng, Xianmin; Chen, Jia; Liu, Ying et al. (2004) BG01V: a variant human embryonic stem cell line which exhibits rapid growth after passaging and reliable dopaminergic differentiation. Restor Neurol Neurosci 22:421-8
Zeng, Xianmin; Miura, Takumi; Luo, Yongquan et al. (2004) Properties of pluripotent human embryonic stem cells BG01 and BG02. Stem Cells 22:292-312
Schulz, Thomas C; Noggle, Scott A; Palmarini, Gail M et al. (2004) Differentiation of human embryonic stem cells to dopaminergic neurons in serum-free suspension culture. Stem Cells 22:1218-38
Brimble, Sandii N; Zeng, Xianmin; Weiler, Deborah A et al. (2004) Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to August 9, 2001. Stem Cells Dev 13:585-97