The nematode, Caenorhabditis elegans, has been identified as a """"""""high connectivity"""""""" animal for cellular development. This very small, simple animal has been widely adopted for experimental study due to its excellent genetics and accessible cell biology. The complete genome of the worm has been published, and most of the adult anatomy has been reconstructed from electron micrographs of serial thin sections. However, EM techniques are not widely utilized by the C. elegans community, and most previous EM data are not easily accessed. The Center for C. elegans Anatomy concentrates on extending the EM methods on C. elegans, particularly to 1) study cellular changes during normal development and in mutant animals, and 2) conduct EM-immunocytochemical and histochemical studies to localize antigens or molecules at the ultrastructural level. Such studies are being conducted in close collaboration with NIH- and NSF-funded laboratories, in fulfillment of their existing experimental aims. In addition, the Center is 3) publishing detailed information regarding the anatomy of the nematode in the form of annotated TEM and light micrographs. Information is available on the Internet on our WormAtlas and Wormlmage web sites, by ftp, on CDs and DVDs, and now in book form in the C. elegans Atlas. Where necessary, the Center is 4) testing new EM methods for C. elegans, and conducting new EM surveys of the wild type anatomy at key developmental stages to supplement the Atlas, and to fill gaps in our general knowledge. The Center also provides 5) training in TEM methods so that more C. elegans labs can conduct their own EM studies. This R24 application was first funded and the Center opened in Feb. 1998;we request five years of funding to continue building our capabilities to serve the scientific community. Many disease-related gene sequences are known in the C. elegans genome;the TEM pathology of genetic mutations in nematode is often helpful in discovering the function of equivalent gene products in man. Studies in this laboratory have uncovered basic mechanisms related to development of the brain, and explored genes involved in several forms of cell death, including apoptosis, necrosis and autophagy.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Resource-Related Research Projects (R24)
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National Center for Research Resources Initial Review Group (RIRG)
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Chang, Michael
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Albert Einstein College of Medicine
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Goupil, Eugénie; Amini, Rana; Hall, David H et al. (2017) Actomyosin contractility regulators stabilize the cytoplasmic bridge between the two primordial germ cells during Caenorhabditis elegans embryogenesis. Mol Biol Cell 28:3789-3800
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Zhang, Jingyan; Li, Xia; Jevince, Angela R et al. (2013) Neuronal target identification requires AHA-1-mediated fine-tuning of Wnt signaling in C. elegans. PLoS Genet 9:e1003618
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Zhang, Hongjie; Kim, Ahlee; Abraham, Nessy et al. (2012) Clathrin and AP-1 regulate apical polarity and lumen formation during C. elegans tubulogenesis. Development 139:2071-83
Hall, David H; Hartwieg, Erika; Nguyen, Ken C Q (2012) Modern electron microscopy methods for C. elegans. Methods Cell Biol 107:93-149
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Abdus-Saboor, Ishmail; Mancuso, Vincent P; Murray, John I et al. (2011) Notch and Ras promote sequential steps of excretory tube development in C. elegans. Development 138:3545-55
McGee, Matthew D; Weber, Darren; Day, Nicholas et al. (2011) Loss of intestinal nuclei and intestinal integrity in aging C. elegans. Aging Cell 10:699-710
Neumann, Brent; Nguyen, Ken C Q; Hall, David H et al. (2011) Axonal regeneration proceeds through specific axonal fusion in transected C. elegans neurons. Dev Dyn 240:1365-72

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