Abstract

The aim of this application is to make the lines of human ES cells listed on the NIH Human ES cells registry available to the research community worldwide.
We aim to be able to distribute 200 aliquots of human ES cells (each containing at least 2x10(6) cell) during the first year, and to double the production and distribution rate during the second year. We will also provide training to recipients which will include: lab manual, hands-on training and a web site support. To ensure the scale-up of high quality human ES cells under tight quality control processes (Good Laboratory Practice), we wish to establish a new fully-equipped laboratory and increase the number of the well-trained biologists. The team will also be involved in basic research aiming at improving human ES-cells. And to explore the variation in growth requirements and differentiation capacity of human ES cell lines. This will significantly increase the potential of research applications of human ES cells and the rate of discoveries of potential human ES cell-derived therapies for treating some of the most common and devastating human diseases.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Resource-Related Research Projects (R24)
Project #
5R24RR018405-04
Application #
7128140
Study Section
Special Emphasis Panel (ZDK1-GRB-B (M2))
Program Officer
Beck, Lawrence A
Project Start
2002-09-30
Project End
2008-08-31
Budget Start
2006-09-01
Budget End
2007-08-31
Support Year
4
Fiscal Year
2006
Total Cost
$237,311
Indirect Cost

  Institution

Name
Technion-Israel Institute of Technology
Department
Type
DUNS #
600133854
City
Haifa
State
Country
Israel
Zip Code
32000

  Publications

Amit, Michal; Itskovitz-Eldor, Joseph (2009) Embryonic stem cells: isolation, characterization and culture. Adv Biochem Eng Biotechnol 114:173-84
Hall, Lisa L; Byron, Meg; Butler, John et al. (2008) X-inactivation reveals epigenetic anomalies in most hESC but identifies sublines that initiate as expected. J Cell Physiol 216:445-52
International Stem Cell Initiative; Adewumi, Oluseun; Aflatoonian, Behrouz et al. (2007) Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Nat Biotechnol 25:803-16
Amit, Michal; Itskovitz-Eldor, Joseph (2006) Feeder-free culture of human embryonic stem cells. Methods Enzymol 420:37-49
Amit, Michal; Itskovitz-Eldor, Joseph (2006) Sources, derivation, and culture of human embryonic stem cells. Semin Reprod Med 24:298-303
Amit, Michal; Winkler, Monica E; Menke, Sandra et al. (2005) No evidence for infection of human embryonic stem cells by feeder cell-derived murine leukemia viruses. Stem Cells 23:761-71
Gerecht-Nir, Sharon; Cohen, Smadar; Itskovitz-Eldor, Joseph (2004) Bioreactor cultivation enhances the efficiency of human embryoid body (hEB) formation and differentiation. Biotechnol Bioeng 86:493-502