The plan of this research is to identify the receptors on macrophages which are involved in the phagocytosis of leishmania promastigotes. Our hypothesis is that the initial binding mechanism determines the parasites' intracellular fate. We have identified two different mechanisms by which macrophages bind leishmania (types I & II), and have preliminary evidence identifying the macrophage receptors involved in each. We will quantitate the two binding mechanisms in a number of model cell systems, including the activated macrophage, the Kupffer cell and selected macrophage-like cell lines. We propose to demonstrate that type I leishmania binding is mediated by the macrophage CR3, that this binding triggers a respiratory burst, and restricts the intracellular growth of leishmania. We also propose to demonstrate that type II leishmania binding is mediated by the macrophage CR1, that binding to this receptor does not trigger the respiratory burst, and that this receptor is permissive for leishmania growth.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
7R29AI024313-03
Application #
3453865
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1988-05-01
Project End
1991-11-30
Budget Start
1988-05-01
Budget End
1988-11-30
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Temple University
Department
Type
Schools of Medicine
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19122