Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29AI032085-06
Application #
2066989
Study Section
Bacteriology and Mycology Subcommittee 2 (BM)
Project Start
1992-09-01
Project End
1997-07-31
Budget Start
1996-08-01
Budget End
1997-07-31
Support Year
6
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Cincinnati
Department
Genetics
Type
Schools of Medicine
DUNS #
City
Cincinnati
State
OH
Country
United States
Zip Code
45221
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Hassett, D J; Howell, M L; Sokol, P A et al. (1997) Fumarase C activity is elevated in response to iron deprivation and in mucoid, alginate-producing Pseudomonas aeruginosa: cloning and characterization of fumC and purification of native fumC. J Bacteriol 179:1442-51
Hassett, D J; Howell, M L; Ochsner, U A et al. (1997) An operon containing fumC and sodA encoding fumarase C and manganese superoxide dismutase is controlled by the ferric uptake regulator in Pseudomonas aeruginosa: fur mutants produce elevated alginate levels. J Bacteriol 179:1452-9
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Hassett, D J; Sokol, P A; Howell, M L et al. (1996) Ferric uptake regulator (Fur) mutants of Pseudomonas aeruginosa demonstrate defective siderophore-mediated iron uptake, altered aerobic growth, and decreased superoxide dismutase and catalase activities. J Bacteriol 178:3996-4003
Brown, S M; Howell, M L; Vasil, M L et al. (1995) Cloning and characterization of the katB gene of Pseudomonas aeruginosa encoding a hydrogen peroxide-inducible catalase: purification of KatB, cellular localization, and demonstration that it is essential for optimal resistance to hydrogen peroxide. J Bacteriol 177:6536-44
DeVries, C A; Hassett, D J; Flynn, J L et al. (1995) Genetic linkage in Pseudomonas aeruginosa of algT and nadB: mutation in nadB does not affect NAD biosynthesis or alginate production. Gene 156:63-7
Hassett, D J; Schweizer, H P; Ohman, D E (1995) Pseudomonas aeruginosa sodA and sodB mutants defective in manganese- and iron-cofactored superoxide dismutase activity demonstrate the importance of the iron-cofactored form in aerobic metabolism. J Bacteriol 177:6330-7
Hassett, D J; Woodruff, W A; Wozniak, D J et al. (1993) Cloning and characterization of the Pseudomonas aeruginosa sodA and sodB genes encoding manganese- and iron-cofactored superoxide dismutase: demonstration of increased manganese superoxide dismutase activity in alginate-producing bacteria. J Bacteriol 175:7658-65