In this proposal, a young investigator wishes to study if proteins involved in T cell activation might be used in the HIV-1 reverse transcription. To facilitate her studies, she has obtained preliminary data demonstrating that T cell proteins bind to biotinylated HIV-1 single stranded DNA and RNA constructs using a non-radioactive adaptation of the electrophoretic mobility shift assay. Using this technique, the investigator proposes to investigate the interaction of HIV-1 reverse transcription intermediates with T cell proteins to test the hypothesis that early and efficient reverse transcription of HIV-1 ssDNA intermediates may assist HIV-1 in the infection process by providing a binding site for host cell proteins involved in pre-integration or integration events. Towards this goal, the investigator proposes the following Specific Aims:
Specific Aim 1 : To delineate the specific sites on HIV-1 RNA, single stranded DNA, RNA DNA hybrid constructs interacting with T cell proteins.
Specific Aim 2 : To investigate interactions between HIV-1 reverse transcription intermediates and the HIV-1 integrase protein
| Ludwig, Linda B; Ambrus Jr, Julian L; Krawczyk, Kristie A et al. (2006) Human Immunodeficiency Virus-Type 1 LTR DNA contains an intrinsic gene producing antisense RNA and protein products. Retrovirology 3:80 |
