Unspliced retroviral RNA is used for both translation of structural proteins and for encapsidation into virions. Although much is now known about how unspliced HIV RNA exists in the nucleus, little is known about the mechanism of trafficking of the RNA between the compartments for translation or encapsidation once the RNA reaches the cytoplasm. This application seeks to test the hypothesis that HIV-1 unspliced RNA forms two functionally distinct pools that are determined by viral sequences: one pool for mRNA translation, and a second pool for encapsidation. The first specific aim of this application is to characterize the metabolism of RNA expressed from simplified HIV-1 genomes that contain distinct elements to facilitate cytoplasmic transport of unspliced RNA (such as Rev, the CTE from MPMV, and an element in the HSV tk gene). The second specific aim is to define the cis and trans-acting domain that modulate trafficking of HIV-1 RNA in the cytoplasm.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29AI040851-05
Application #
6373599
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Program Officer
Sharma, Opendra K
Project Start
1997-09-30
Project End
2003-08-31
Budget Start
2001-09-01
Budget End
2003-08-31
Support Year
5
Fiscal Year
2001
Total Cost
$106,840
Indirect Cost
Name
Ohio State University
Department
Veterinary Sciences
Type
Schools of Veterinary Medicine
DUNS #
098987217
City
Columbus
State
OH
Country
United States
Zip Code
43210