Abstract

Thrombospondin (TSP) is a newly described protein of the extracellular matrix (ECM). The purpose of this project is to investigate its function in the epidermis. The initial approach to this problem will be via an in vitro model. Pure human keratinocyte cultures in a defined, serum-free medium will be used to investigate the effects of numerous variables (cellular, culture, substrate, external mediators) on the synthesis and distribution of TSP by keratinocytes. Immunohistochemical techniques at both the light and electron microscopic levels will be used to investigate the distribution of TSP and its spacial relationship to other ECM proteins in normal skin, healing wounds, and some inflammatory and bullous skin diseases. Through these studies the biological function of TSP in the epidermis should be illuminated.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29AR038508-06
Application #
3456773
Study Section
Special Emphasis Panel (ADDK)
Project Start
1990-03-01
Project End
1992-06-30
Budget Start
1990-07-01
Budget End
1992-06-30
Support Year
6
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
University of Colorado Denver
Department
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045