We have identified a number of tyrosine phosphoproteins in RSV-infected cells. These proteins were identified using anti-phosphotyrosine antibodies; many of them are also phosphorylated with [gamma-32P] ATP in situ in the cytoskeletal framework remaining after extraction of cells with Triton X-100. In collaboration with Professor P. M. Comoglio (University of Turin), we have begun to purify some of these proteins, with the intention of producing antibodies to them. We have already succeeded in the process of subcloning positive primary hybridoma cultures obtained from this mouse for the production of monoclonal antibodies. We wish to continue this collaborative effort.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29CA047098-04
Application #
3458811
Study Section
Experimental Virology Study Section (EVR)
Project Start
1988-12-01
Project End
1993-11-30
Budget Start
1991-12-01
Budget End
1992-11-30
Support Year
4
Fiscal Year
1992
Total Cost
Indirect Cost
Name
University of Texas-Dallas
Department
Type
Schools of Arts and Sciences
DUNS #
City
Richardson
State
TX
Country
United States
Zip Code
75080