Retroviruses have a high mutation frequency. However, until recently protocols had not been developed for measuring retroviral mutation rates, that is, the mutation frequency in a single cycle of retrovirus replication. Using a recently developed protocol (Dougherty and Temin, 1986) experiments are proposed to determine retrovirus mutation rates for defined mutations, such as base substitutions, insertions, deletions, and inversions. Experiments measuring recombination mutation rates are also proposed. Mutation rates will be determined using spleen necrosis virus (SNV)-, murine leukemia virus (MLV)-, and human immunodeficiency virus (HIV)-based retroviral vector systems. Studies are also proposed to determine mutation rates using replication-competent retroviruses.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29CA050777-05
Application #
3459656
Study Section
Special Emphasis Panel (ARR (V1))
Project Start
1989-06-15
Project End
1994-05-31
Budget Start
1993-06-01
Budget End
1994-05-31
Support Year
5
Fiscal Year
1993
Total Cost
Indirect Cost
Name
University of Medicine & Dentistry of NJ
Department
Type
Schools of Medicine
DUNS #
622146454
City
Piscataway
State
NJ
Country
United States
Zip Code
08854
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