Myeloid leukemias result from the dysregulation of proliferation and differentiation. Hematopoietic growth factors and their receptors play a critical role in lineage commitment and differentiation of myeloid progenitor cells. Egr-1, or early growth response gene-1 is an immediate early response gene which is required for terminal differentiation of myeloid cells. In response to differentiation agents, the induction of egr-1 is rapid in response to granulocyte-colony stimulating factor (G-CSF) or 12-0 Tetradecanoylphorbol-13-acetate (TPA) and myeloid leukemic cell lines. The overall goal of this FIRST Award proposal is to use a molecular and biochemical approach to define the transcriptional regulation of egr-1 by a specific DNA-binding proteins activated by myeloid-specific differentiating agents. The myeloid cell lines will be used as a tool to study the transcriptional regulation of egr-1 by growth factors and agents which induce differentiation. The myeloid leukemic cell lines, 32Dc13, differentiatesinto granulocyte but does not proliferate in the presence of G-CSF. TF-1, a human myeloid leukemic cell line, differentiates to macrophages in response to TPA.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
3R29CA068221-04S1
Application #
6076164
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1996-07-01
Project End
2001-04-30
Budget Start
1999-05-01
Budget End
2000-04-30
Support Year
4
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of California Los Angeles
Department
Pediatrics
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095