Identification of novel oncogenes is an important aspect of cancer research. Potential oncogenes are being identified based on their genetic alterations or abnormal gene expression patterns. It is hypothesized that some signal transducing proteins are only transiently required in tumorigenesis, their expression patterns return to normal in tumor cells, and therefore, their oncogenicity is unrecognized. This hypothesis is consistent with a notion that oncogenes generally function through transient activation mechanisms in normal differentiation process. The hypothesis is also supported by the investigator's recent observations made on CD3 transgenic mice: Although high levels of CD3 transgene expression in early thymocytes were necessary to induce lymphomas, this transgene expression was not detected in the tumor cells. It is apparent that the oncogenicity of CD3 would be unrecognized if only the tumor cells were analyzed. It is proposed to test the hypothesis that transient overexpression of oncogenic signal transducing proteins can induce tumor. The strategy is to control the timing of oncogene expression in transgenic mice: To start oncogene expression using a tetracycline-inducible system and to stop oncogene expression by deletion of the oncogene using an interferon-inducible, Cre-loxP-mediated deletion system. Three genes, CD3 , lck, and Shc, will be induced for transient overexpression in transgenic mice which will then be monitored for lymphomagenesis. These genes are chosen based on following reasons: 1) They are transiently activated in signal transduction cascades in normal cells; 2) Both CD3 and lck can rapidly induce T cell lymphomas when overexpressed early in thymocyte development; 3) Shc is a part of the CD3 and lck involved signal pathway, and its overexpression in vitro can induce cell transformation; 4) They represent three types of signal transducing molecules: docking sites, kinases, and adapters, respectively.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29CA074233-02
Application #
2700744
Study Section
Metabolic Pathology Study Section (MEP)
Program Officer
Mccarthy, Susan A
Project Start
1997-05-15
Project End
1999-04-30
Budget Start
1998-05-01
Budget End
1999-04-30
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02215