Abstract

The aims are: (1) to characterize the role of lipids in signal transduction by ECM molecules for tumor cell pseudopod protrusion and migration; (2) to determine the response of intracellular calcium cations in tumor cells to lipid-mediated ECM molecule stimulation; (3) to examine the response of the cytoskeletal actin network upon lipid- mediated ECM molecule stimulation during the two phase pseudopod protrusion; and (4) to construct a two phase pseudopod model integrating engineering sciences with cell biology and derive mechanistic parameters that may affect tumor cell migration.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29CA076434-04
Application #
6329017
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Program Officer
Ault, Grace S
Project Start
1997-12-15
Project End
2002-11-30
Budget Start
2000-12-01
Budget End
2001-11-30
Support Year
4
Fiscal Year
2001
Total Cost
$98,232
Indirect Cost

  Institution

Name
Pennsylvania State University
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
City
University Park
State
PA
Country
United States
Zip Code
16802

  Publications

Slattery, Margaret J; Dong, Cheng (2003) Neutrophils influence melanoma adhesion and migration under flow conditions. Int J Cancer 106:713-22
Hodgson, Louis; Henderson, Andrew J; Dong, Cheng (2003) Melanoma cell migration to type IV collagen requires activation of NF-kappaB. Oncogene 22:98-108
Dong, Cheng; Slattery, Margaret J; Rank, Bradley M et al. (2002) In vitro characterization and micromechanics of tumor cell chemotactic protrusion, locomotion, and extravasation. Ann Biomed Eng 30:344-55
Hodgson, L; Dong, C (2001) [Ca2+]i as a potential downregulator of alpha2beta1-integrin-mediated A2058 tumor cell migration to type IV collagen. Am J Physiol Cell Physiol 281:C106-13
Hodgson, L; Qiu, W; Dong, C et al. (2000) Use of green fluorescent protein-conjugated beta-actin as a novel molecular marker for in vitro tumor cell chemotaxis assay. Biotechnol Prog 16:1106-14
Hodgson, L; Kohn, E C; Dong, C (2000) Extracellular lipid-mediated signaling in tumor-cell activation and pseudopod protrusion. Int J Cancer 88:593-600
You, J; Mastro, A M; Dong, C (1999) Application of the dual-micropipet technique to the measurement of tumor cell locomotion. Exp Cell Res 248:160-71