The goals of the present study are to examine mechanisms by which autoantibodies to double-stranded DNA can localize to intracellular and specifically intranuclear targets in affected cells. The Principal Investigator has identified an anti-DNA antibody, mAb 3E10, that will selectively bind to kidney tubular epithelial cells and nuclei. The goals of the present study, are to characterize and detail the autoantibody determinants that are necessary for entry across the plasma membrane into the affected cells using site directed mutagenesis and chimeric antibodies and then to characterize the antibody determinants that are utilized for transport into the nucleus of the cell, and compare the two. The other Specific Aim is to directly label this antibody in order to develop systems that are useful for the identification of potential inhibitors of intracellular and intranuclear localization.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29DK050684-05
Application #
6177484
Study Section
Pathology A Study Section (PTHA)
Program Officer
Hirschman, Gladys H
Project Start
1996-08-05
Project End
2002-07-31
Budget Start
2000-08-01
Budget End
2002-07-31
Support Year
5
Fiscal Year
2000
Total Cost
$90,737
Indirect Cost
Name
University of California Los Angeles
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095