Abstract

Dr. Milgram proposes experiments aimed at increasing understanding of mechanisms of protein sorting in neuroendocrine cells. The following two questions will be addressed: (1) What are the proteins that interact with routing determinants in the peptidylglycine alpha-amidating monooxygenase (PAM) cytosolic domain? (2) What is the role of the novel PAM cytosolic domain binding protein, BP1?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29DK050744-05
Application #
6177494
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Program Officer
Haft, Carol Renfrew
Project Start
1996-09-30
Project End
2001-07-31
Budget Start
2000-08-01
Budget End
2001-07-31
Support Year
5
Fiscal Year
2000
Total Cost
$92,882
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Physiology
Type
Schools of Medicine
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Scott, Robert O; Thelin, William R; Milgram, Sharon L (2002) A novel PDZ protein regulates the activity of guanylyl cyclase C, the heat-stable enterotoxin receptor. J Biol Chem 277:22934-41
Schmidt, P H; Dransfield, D T; Claudio, J O et al. (1999) AKAP350, a multiply spliced protein kinase A-anchoring protein associated with centrosomes. J Biol Chem 274:3055-66
Kachinsky, A M; Froehner, S C; Milgram, S L (1999) A PDZ-containing scaffold related to the dystrophin complex at the basolateral membrane of epithelial cells. J Cell Biol 145:391-402
Mohler, P J; Kreda, S M; Boucher, R C et al. (1999) Yes-associated protein 65 localizes p62(c-Yes) to the apical compartment of airway epithelia by association with EBP50. J Cell Biol 147:879-90
Smith, F D; Oxford, G S; Milgram, S L (1999) Association of the D2 dopamine receptor third cytoplasmic loop with spinophilin, a protein phosphatase-1-interacting protein. J Biol Chem 274:19894-900
Chen, L; Johnson, R C; Milgram, S L (1998) P-CIP1, a novel protein that interacts with the cytosolic domain of peptidylglycine alpha-amidating monooxygenase, is associated with endosomes. J Biol Chem 273:33524-32
Short, D B; Trotter, K W; Reczek, D et al. (1998) An apical PDZ protein anchors the cystic fibrosis transmembrane conductance regulator to the cytoskeleton. J Biol Chem 273:19797-801