Abstract

Angler fish peptide YG (APY) which is 64% homologous to both neuropeptide Y (NPY) and peptide YY (PYY) will be synthesized by solid phase method. The peptide will be tested for PYY and NPY-like activities, namely the effects on pancreatic secretion and on intestinal blood flow in dogs. Amidated form of APY will be synthesized to investigate whether the APY amide will have the same biological activity as the glycine extended form, the intact hormone. Minimum sequence requirements for activity will be examined by the synthesis and investigation of the biological properties of selected fragments of APY. Antibodies raised against the intact hormone will be used to determine whether APY-like immunoreactive peptide is present in mammals. Selected fragments and analogs of PYY and NPY will be synthesized to determine the sites involved in receptor binding and biological activity. Results of these experiments will form the basis for the development of agonistic and antagonistic peptides for PYY and NPY.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29GM038601-04
Application #
3466328
Study Section
Bio-Organic and Natural Products Chemistry Study Section (BNP)
Project Start
1988-04-01
Project End
1993-03-31
Budget Start
1991-04-01
Budget End
1992-03-31
Support Year
4
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
University of Cincinnati
Department
Type
Schools of Medicine
DUNS #
City
Cincinnati
State
OH
Country
United States
Zip Code
45221

  Publications

Bjenning, C; Hazon, N; Balasubramaniam, A et al. (1993) Distribution and activity of dogfish NPY and peptide YY in the cardiovascular system of the common dogfish. Am J Physiol 264:R1119-24
Sheriff, S; Balasubramaniam, A (1992) Inhibitory and stimulatory effects of neuropeptide Y(17-36) on rat cardiac adenylate cyclase activity. Structure-function studies. J Biol Chem 267:4680-5
Inui, A; Okita, M; Miura, M et al. (1992) Solubilization of high affinity peptide-YY receptors from porcine brain. Endocrinology 130:2120-8
Balasubramaniam, A; Rigel, D F; Chance, W T et al. (1992) Central and peripheral effects of sculpin pancreatic polypeptide and anglerfish peptide Y in rats. Pept Res 5:106-9
Millar, B C; Weis, T; Piper, H M et al. (1991) Positive and negative contractile effects of neuropeptide Y on ventricular cardiomyocytes. Am J Physiol 261:H1727-33
Conlon, J M; Balasubramaniam, A; Hazon, N (1991) Structural characterization and biological activity of a neuropeptide Y-related peptide from the dogfish, Scyliorhinus canicula. Endocrinology 128:2273-9
Chance, W T; Balasubramaniam, A; Zhang, F S et al. (1991) Anorexia following the intrahypothalamic administration of amylin. Brain Res 539:352-4
Balasubramaniam, A; Renugopalakrishnan, V; Stein, M et al. (1991) Syntheses, structures and anorectic effects of human and rat amylin. Peptides 12:919-24
Balasubramaniam, A; Sheriff, S; Rigel, D F et al. (1990) Characterization of neuropeptide Y binding sites in rat cardiac ventricular membranes. Peptides 11:545-50
Balasubramaniam, A; Sheriff, S (1990) Neuropeptide Y (18-36) is a competitive antagonist of neuropeptide Y in rat cardiac ventricular membranes. J Biol Chem 265:14724-7

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