Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29GM039334-02
Application #
3466690
Study Section
Biochemistry Study Section (BIO)
Project Start
1988-02-01
Project End
1989-08-31
Budget Start
1989-02-01
Budget End
1989-08-31
Support Year
2
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Carnegie-Mellon University
Department
Type
Schools of Arts and Sciences
DUNS #
052184116
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Musial-Siwek, Monika; Jaffee, Marcie B; Imperiali, Barbara (2016) Probing Polytopic Membrane Protein-Substrate Interactions by Luminescence Resonance Energy Transfer. J Am Chem Soc 138:3806-12
Lukose, Vinita; Luo, Lingqi; Kozakov, Dima et al. (2015) Conservation and Covariance in Small Bacterial Phosphoglycosyltransferases Identify the Functional Catalytic Core. Biochemistry 54:7326-34
Whitworth, Garrett E; Imperiali, Barbara (2015) Selective biochemical labeling of Campylobacter jejuni cell-surface glycoconjugates. Glycobiology 25:756-66
Lukose, Vinita; Whitworth, Garrett; Guan, Ziqiang et al. (2015) Chemoenzymatic Assembly of Bacterial Glycoconjugates for Site-Specific Orthogonal Labeling. J Am Chem Soc 137:12446-9
Chang, Michelle M; Imperiali, Barbara; Eichler, Jerry et al. (2015) N-Linked Glycans Are Assembled on Highly Reduced Dolichol Phosphate Carriers in the Hyperthermophilic Archaea Pyrococcus furiosus. PLoS One 10:e0130482
Jaffee, Marcie B; Imperiali, Barbara (2013) Optimized protocol for expression and purification of membrane-bound PglB, a bacterial oligosaccharyl transferase. Protein Expr Purif 89:241-50
Larkin, Angelyn; Chang, Michelle M; Whitworth, Garrett E et al. (2013) Biochemical evidence for an alternate pathway in N-linked glycoprotein biosynthesis. Nat Chem Biol 9:367-73
Hartley, Meredith D; Schneggenburger, Philipp E; Imperiali, Barbara (2013) Lipid bilayer nanodisc platform for investigating polyprenol-dependent enzyme interactions and activities. Proc Natl Acad Sci U S A 110:20863-70
Hartley, Meredith D; Imperiali, Barbara (2012) At the membrane frontier: a prospectus on the remarkable evolutionary conservation of polyprenols and polyprenyl-phosphates. Arch Biochem Biophys 517:83-97
Jaffee, Marcie B; Imperiali, Barbara (2011) Exploiting topological constraints to reveal buried sequence motifs in the membrane-bound N-linked oligosaccharyl transferases. Biochemistry 50:7557-67

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