The principal goal of this proposal is the isolation of monoclonal antibodies with catalytic activities. Such antibodies will have potential for furthering our understanding of enzyme catalysis, and ultimately may be useful in the treatment of diseases; furthermore, numerous biochemical applications can be envisioned. Specifically, by immunizing with a variety of analogues, we will attempt to isolate antibodies with sequence-specific protease activity. Given the recent successes in isolating antibodies that cleave carbonates and esters, the hydrolysis of a peptide bond is a reasonable goal; furthermore, sequence specificity can easily be introduced by raising antibodies against peptides containing the various analogues proposed. In addition, we will pursue the isolation of antibodies capable of catalyzing simpler reactions that will not require acid-base chemistry for catalysis to occur.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29GM039758-04
Application #
3466940
Study Section
Bio-Organic and Natural Products Chemistry Study Section (BNP)
Project Start
1988-04-01
Project End
1993-03-31
Budget Start
1991-04-01
Budget End
1992-03-31
Support Year
4
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Amherst College
Department
Type
Schools of Arts and Sciences
DUNS #
City
Amherst
State
MA
Country
United States
Zip Code
01002
Smith, R M; Yuan, P; Weiner, D P et al. (1994) An approach to sequence-specific antibody proteases. The use of haptens mimicking both a transition state and a distorted ground state. Appl Biochem Biotechnol 47:329-42;discussion 342-3