This application seeks to examine regulation of myometrial OTR expression in a murine model. Specifically, the investigator will 1) define the molecular organization and regulatory regions of the mouse OTR gene by genomic cloning, DNA sequence analysis, and identification of putative transcription factor consensus sequences; 2) test putative cis-acting OTR gene regulatory elements in vitro via quantitation of OTR-luciferase reporter gene expression in transfected primary myometrial myocytes and cell lines, and modulation by estrogenic steroids; and 3) define the genetic information required for spatial, temporal, and estrogen (E) regulation of the native OTR gene in transgenic mice that express an OTR-beta-galactosidase reporter gene.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29HD033775-05
Application #
6181718
Study Section
Human Embryology and Development Subcommittee 1 (HED)
Program Officer
Ilekis, John V
Project Start
1996-09-16
Project End
2002-08-31
Budget Start
2000-09-01
Budget End
2002-08-31
Support Year
5
Fiscal Year
2000
Total Cost
$103,014
Indirect Cost
Name
Children's Hospital Boston
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02115