This revised FIRST Award application seeks to better understand endothelial cell (EC) adhesion and migration as a function of flow and cytokines. The protein and mRNA expression of endothelial GPIb and VnR will be measured as a function of shear stress in the presence and absence of cytokines tumor necrosis factor alpha and interferon gamma. The relative contribution of EC GPIb/IX and VnR to adhesion and migration will be studied under conditions of variable shear stress and in the presence and absence of cytokines. Finally, the role of the EC cytoskeleton in GPIb/IX-dependent EC adhesion and migration will be studied under conditions of variable shear stress and in the presence and absence of cytokines.
| Lian, J; Marcinkiewicz, C; Niewiarowski, S et al. (2001) Extracellular signal-regulated kinase (ERK) activation is required for GP Ibalpha-dependent endothelial cell migration. Thromb Haemost 86:1555-62 |
| Cai, G; Lian, J; Shapiro, S S et al. (2000) Evaluation of endothelial cell migration with a novel in vitro assay system. Methods Cell Sci 22:107-14 |
| Beacham, D A; Lian, J; Wu, G et al. (1999) Arterial shear stress stimulates surface expression of the endothelial glycoprotein Ib complex. J Cell Biochem 73:508-21 |
| Lian, J; Guoping, C; Shapiro, S S et al. (1999) Glycoprotein Ibalpha can mediate endothelial cell migration on von Willebrand factor-containing substrata. Exp Cell Res 252:114-22 |
