The proposal addresses the problem of how platelet surface glycoprotein Ib-IX (the receptor for von Willebrand factor) signals to activate the platelet following GPIb-vWf interaction. Progress has been made recently by the PI in identifying and characterizing a protein (29kDa) that is associated with GPIb-IX. The protein has been identified as P29, a modifier of protein kinase C, also termed a x-form of 14-3-3 which has phospholipase A2 activity. The proposal sets out to characterize the interaction between 14-3-3 and GP Ib-IX and to characterize the functions of 14-3-3. Specifically, four aims are proposed: first, further characterize the interaction between GPIb-IX and 14-3-3, second, examine the functions of P29 and how it is modulated by ligand binding to GPIIb-IX, third, characterize and express the phosholipase activity of P29, fourth, characterize the structural basis of the interaction between GPIb-IX and P29.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29HL052547-05
Application #
2702252
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1995-05-01
Project End
2000-04-30
Budget Start
1998-05-01
Budget End
1999-04-30
Support Year
5
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Illinois at Chicago
Department
Pharmacology
Type
Schools of Medicine
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612
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