Platelet P2T- purinergic receptors are unique among P2-purinergic receptors since ADP is the preferred agonist and ATP an antagonist. Preliminary studies indicate that at least two cell lines that express P2T-purinergic receptors have been identified.
The specific aims are: 1) To identify a model cell line for the study of the P2T-purinergic receptors. 2) To determine the signalling mechanism(s) and the G-protein(s) and phospholipase C involved in the activation of the P2T-purinergic receptors. 3) To clone the cDNA encoding the receptor from a human megakaryocytic cell line. 4) To stably express the cloned P2T- purinergic receptor(s) to establish its signalling mechanisms and to provide a model for eventual development of more potent and specific agonists and antagonists.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29HL054889-03
Application #
2460149
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1995-09-01
Project End
2000-07-31
Budget Start
1997-08-01
Budget End
1998-07-31
Support Year
3
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Pharmacology
Type
Schools of Medicine
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599