Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29NS033740-02
Application #
2272714
Study Section
Toxicology Subcommittee 2 (TOX)
Program Officer
Kitt, Cheryl A
Project Start
1995-07-01
Project End
2000-05-31
Budget Start
1996-06-01
Budget End
1997-05-31
Support Year
2
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Massachusetts Dartmouth
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
City
North Dartmouth
State
MA
Country
United States
Zip Code
02747
Cai, Shuowei; Singh, Bal Ram (2004) A distinct utility of the amide III infrared band for secondary structure estimation of aqueous protein solutions using partial least squares methods. Biochemistry 43:2541-9
Fu, Fen-Ni; Busath, David D; Singh, Bal Ram (2002) Spectroscopic analysis of low pH and lipid-induced structural changes in type A botulinum neurotoxin relevant to membrane channel formation and translocation. Biophys Chem 99:17-29
Cai, S; Singh, B R (2001) Role of the disulfide cleavage induced molten globule state of type a botulinum neurotoxin in its endopeptidase activity. Biochemistry 40:15327-33
Cai, S; Singh, B R (2001) A correlation between differential structural features and the degree of endopeptidase activity of type A botulinum neurotoxin in aqueous solution. Biochemistry 40:4693-702
Puffer, E B; Lomneth, R B; Sarkar, H K et al. (2001) Differential roles of developmentally distinct SNAP-25 isoforms in the neurotransmitter release process. Biochemistry 40:9374-8
Li, L; Singh, B R (2000) Role of zinc binding in type A botulinum neurotoxin light chain's toxic structure. Biochemistry 39:10581-6
Li, L; Binz, T; Niemann, H et al. (2000) Probing the mechanistic role of glutamate residue in the zinc-binding motif of type A botulinum neurotoxin light chain. Biochemistry 39:2399-405
Li, L; Singh, B R (2000) Spectroscopic analysis of pH-induced changes in the molecular features of type A botulinum neurotoxin light chain. Biochemistry 39:6466-74
Sharma, S K; Singh, B R (2000) Immunological properties of Hn-33 purified from type A Clostridium botulinum. J Nat Toxins 9:357-62
Cai, S; Sarkar, H K; Singh, B R (1999) Enhancement of the endopeptidase activity of botulinum neurotoxin by its associated proteins and dithiothreitol. Biochemistry 38:6903-10

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