This application's major objective is to test the efficacy of a potential microbicidal agent to prevent transmission of HIV to women. Such an effective agent is urgently needed to combat the predominant rise globally of new HIV infections in women. Based on promising preliminary results in vitro, the specific aim of this application is to test in vivo the efficacy of glycerol monolaurate (GML) to prevent intravaginal transmission of simian immunodeficiency virus (SIV) to rhesus macaques, a highly relevant nonhuman primate model of sexual mucosal transmission of HIV. Treated or control monkeys will be infected and biopsy and necropsy specimens will analyzed by immunohistochemical and in situ hybridization methods and viral loads in blood to assess the efficacy of GML in preventing transmission or altering viral replication in cervicovaginal and ? lymphatic tissues. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Exploratory/Developmental Grants Phase II (R33)
Project #
4R33AI071976-03
Application #
7664165
Study Section
Special Emphasis Panel (NSS)
Program Officer
Turpin, Jim A
Project Start
2006-09-20
Project End
2011-08-31
Budget Start
2008-09-11
Budget End
2009-08-31
Support Year
3
Fiscal Year
2008
Total Cost
$463,016
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
Haase, Ashley T; Rakasz, Eva; Schultz-Darken, Nancy et al. (2015) Glycerol Monolaurate Microbicide Protection against Repeat High-Dose SIV Vaginal Challenge. PLoS One 10:e0129465
Haase, Ashley T (2010) Targeting early infection to prevent HIV-1 mucosal transmission. Nature 464:217-23