Abstract

) This project has two broad goals. The first is to develop CD-tagging technology into a robust tool for comprehensive analysis of the proteome of cultured cells, including primary tumor cells. The second is to develop discovery and analysis technologies that efficiently search CD-tagged cell libraries to identify and analyze genes and proteins that may have value in cancer diagnostics and therapeutics. Within this second goal we will focus on two areas: identifying cell surface receptors that trigger growth arrest or apoptosis, and identifying proteins whose levels rise in response to myc oncogene activation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Exploratory/Developmental Grants Phase II (R33)
Project #
1R33CA083219-01A1
Application #
6140918
Study Section
Special Emphasis Panel (ZCA1-SRRB-C (J1))
Program Officer
Gallahan, Daniel L
Project Start
2000-05-23
Project End
2003-04-30
Budget Start
2000-05-23
Budget End
2001-04-30
Support Year
1
Fiscal Year
2000
Total Cost
$741,536
Indirect Cost

  Institution

Name
Carnegie-Mellon University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
052184116
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Chen, Xiang; Velliste, Meel; Murphy, Robert F (2006) Automated interpretation of subcellular patterns in fluorescence microscope images for location proteomics. Cytometry A 69:631-40
Huang, Kai; Murphy, Robert F (2004) From quantitative microscopy to automated image understanding. J Biomed Opt 9:893-912
Huang, Kai; Murphy, Robert F (2004) Boosting accuracy of automated classification of fluorescence microscope images for location proteomics. BMC Bioinformatics 5:78
Hu, Yanhua; Murphy, Robert F (2004) Automated interpretation of subcellular patterns from immunofluorescence microscopy. J Immunol Methods 290:93-105
Murphy, Robert F (2004) Automated interpretation of protein subcellular location patterns: implications for early cancer detection and assessment. Ann N Y Acad Sci 1020:124-31
Yi, Fenghua; Jaffe, Ronald; Prochownik, Edward V (2003) The CCL6 chemokine is differentially regulated by c-Myc and L-Myc, and promotes tumorigenesis and metastasis. Cancer Res 63:2923-32
Price, Jeffrey H; Goodacre, Angela; Hahn, Klaus et al. (2002) Advances in molecular labeling, high throughput imaging and machine intelligence portend powerful functional cellular biochemistry tools. J Cell Biochem Suppl 39:194-210
Jarvik, J W; Fisher, G W; Shi, C et al. (2002) In vivo functional proteomics: mammalian genome annotation using CD-tagging. Biotechniques 33:852-4, 856, 858-60 passim
Telmer, C A; Berget, P B; Ballou, B et al. (2002) Epitope tagging genomic DNA using a CD-tagging Tn10 minitransposon. Biotechniques 32:422-4, 426, 428-30
Roques, Edward J S; Murphy, Robert F (2002) Objective evaluation of differences in protein subcellular distribution. Traffic 3:61-5

Showing the most recent 10 out of 12 publications