Abstract

It is proposed to analyze the genetic structure and, in particular, the transforming onc genes and proteins of avian and murine retroviruses and of their cellular prototypes, termed proto-onc genes. Viral onc genes consist of specific elements derived from cellular proto-onc genes as well as from retroviral genes. For this reason, it is now widely believed that proto-onc genes can function as cellular cancer genes upon transcriptional or mutational activation. This project will analyze common and specific properties of corresponding onc genes and proto-onc genes, in order to determine whether and under which conditions proto-onc genes may function as cancer genes. The studies will first involve structural analyses of onc genes and proto-onc genes. Subsequently, the function of structurally defined onc- and proto-onc-gene domains will be analyzed in viral vectors by deletion analyses and by in vitro recombination analyses between the onc genes of cloned retroviruses with corresponding proto-onc genes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Unknown (R35)
Project #
5R35CA039915-05
Application #
3479262
Study Section
(SRC)
Project Start
1985-09-30
Project End
1992-08-31
Budget Start
1989-09-01
Budget End
1990-08-31
Support Year
5
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of California Berkeley
Department
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704

  Publications

Wu, Y; Zhou, H; Duesberg, P (1992) Unmutated proto-src coding region is tumorigenic if expressed from the promoter of Rous sarcoma virus: implications for the gene-mutation hypothesis of cancer. Proc Natl Acad Sci U S A 89:6393-7
Gulino, S E (1992) Chromosome abnormalities and oncogenesis in cat leukemias. Cancer Genet Cytogenet 64:149-57
Duesberg, P H (1992) The role of drugs in the origin of AIDS. Biomed Pharmacother 46:3-15
Duesberg, P H; Schwartz, J R (1992) Latent viruses and mutated oncogenes: no evidence for pathogenicity. Prog Nucleic Acid Res Mol Biol 43:135-204
Duesberg, P H (1992) AIDS acquired by drug consumption and other noncontagious risk factors. Pharmacol Ther 55:201-77
Duesberg, P H; Goodrich, D; Zhou, R P (1991) Cancer genes by non-homologous recombination. Basic Life Sci 57:197-211
Duesberg, P H (1991) AIDS epidemiology: inconsistencies with human immunodeficiency virus and with infectious disease. Proc Natl Acad Sci U S A 88:1575-9
Goodrich, D W; Duesberg, P H (1990) Evidence that retroviral transduction is mediated by DNA not by RNA. Proc Natl Acad Sci U S A 87:3604-8
Duesberg, P H (1990) ""The AIDS debate"". Naturwissenschaften 77:97-102
Duesberg, P H (1990) AIDS: non-infectious deficiencies acquired by drug consumption and other risk factors. Res Immunol 141:5-11

Showing the most recent 10 out of 24 publications