My group has a long?standing interest in understanding the molecular mechanisms that contribute to cancer biology and genome integrity. To provide new insights into the mechanisms of genome integrity we have pioneered the development of DNA curtains, which allow for the direct visualization of hundreds to thousands of individual molecules in real?time by optical microscopy. This experimental platform is applicable to many types of protein?nucleic acid interactions and allows us to address questions about complex biological problems that cannot be addressed through traditional approaches. Our success in with this technology has uniquely positioned the lab to reveal new concepts regarding the mechanisms of genome integrity
Double-stranded DNA breaks (DSBs) can cause gross chromosomal rearrangements, which are the hallmark of all forms of cancer. The overall goal of our research is to study the events that lead to DSB formation and the mechanisms that cells use for DSB repair, in the hope that this research will eventually lead to a better understanding of how might be able to prevent or cure cancer
| De Tullio, Luisina; Kaniecki, Kyle; Greene, Eric C (2018) Single-Stranded DNA Curtains for Studying the Srs2 Helicase Using Total Internal Reflection Fluorescence Microscopy. Methods Enzymol 600:407-437 |
| Crickard, J Brooks; Kaniecki, Kyle; Kwon, YoungHo et al. (2018) Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments. J Biol Chem 293:4191-4200 |
| Crickard, J Brooks; Kaniecki, Kyle; Kwon, YoungHo et al. (2018) Regulation of Hed1 and Rad54 binding during maturation of the meiosis-specific presynaptic complex. EMBO J 37: |
| Crickard, J Brooks; Kaniecki, Kyle; Kwon, Youngho et al. (2018) Meiosis-specific recombinase Dmc1 is a potent inhibitor of the Srs2 antirecombinase. Proc Natl Acad Sci U S A 115:E10041-E10048 |
| Kaniecki, Kyle; De Tullio, Luisina; Greene, Eric C (2018) A change of view: homologous recombination at single-molecule resolution. Nat Rev Genet 19:191-207 |
| Crickard, J Brooks; Greene, Eric C (2018) Biochemical attributes of mitotic and meiotic presynaptic complexes. DNA Repair (Amst) : |
| Zhao, Weixing; Steinfeld, Justin B; Liang, Fengshan et al. (2017) BRCA1-BARD1 promotes RAD51-mediated homologous DNA pairing. Nature 550:360-365 |
| Terakawa, Tsuyoshi; Bisht, Shveta; Eeftens, Jorine M et al. (2017) The condensin complex is a mechanochemical motor that translocates along DNA. Science 358:672-676 |
| Ma, Chu Jian; Kwon, Youngho; Sung, Patrick et al. (2017) Human RAD52 interactions with replication protein A and the RAD51 presynaptic complex. J Biol Chem 292:11702-11713 |
| Erdel, Fabian; Kratz, Katja; Willcox, Smaranda et al. (2017) Telomere Recognition and Assembly Mechanism of Mammalian Shelterin. Cell Rep 18:41-53 |
Showing the most recent 10 out of 25 publications
