Antibiotic resistance emerges when a mutation in a bacterium causes a previously inhibitory concentration of a compound to become survivable. Through the accumulation of mutations conferring varying increases in resistance, already many easy-to-treat infections have become nearly incurable, and are spreading in part anthropogenically. The classical model of resistance evolution, that a resistant mutant has a fitness advantage in the presence of antibiotic use, and so spreads in the population to near-fixation, captures the rise of antibiotic resistance, but on closer inspection fails to explain several critical features of resistance. First, antibiotic resistance rarely reaches fixation in clinical populations; more importantly, sensitivity is higher than the population-genetic models would predict. Second, antibiotic resistance was present, and likely common, in clinical infections before the human use of antibiotics even began. Third, despite the widespread prevalence of antibiotic-producing bacteria in the environment, these same bacteria remain surrounded by sensitive neighbors. For these reasons, we hypothesize that the existing model of resistance evolution is incomplete, and in particular that there exist evolutionary factors in the environment which have a potentially countervailing effect on resistance evolution of similar or greater magnitude to the human use of antibiotics. Here, we will combine evolution experiments in model systems with computational modeling and database mining of sequence data to study the constraints on the evolution of resistance, focusing on two key areas: the role of spatial structure in the evolution of resistance, and the role of selfish genetic elements including phages and parasitic plasmids. Resistance provides an almost ideal model system for the study of microbial evolution; fitness can be well defined, imposed selective pressures can be readily tuned, and can emerge either spontaneously or by horizontal gene transfer. We expect to uncover the evolutionary mechanisms behind the emergence, spread, and limitation of antibiotic resistance.

Public Health Relevance

Antibiotic resistance in bacteria is a public health crisis. Understanding the evolutionary dynamics that result in the emergence or suppression of resistance will open new avenues of approach to antibiotic resistance. For individual patients with long-term infections it would allow the development of new therapies which consider and potentially exploit the evolutionary possibilities of the pathogen, while on the population scale, understanding which broad dynamics are important for resistance to spread or be contained could enable new public health strategies.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Unknown (R35)
Project #
5R35GM133700-02
Application #
9980445
Study Section
Special Emphasis Panel (ZGM1)
Program Officer
Janes, Daniel E
Project Start
2019-08-01
Project End
2024-05-31
Budget Start
2020-06-01
Budget End
2021-05-31
Support Year
2
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Harvard Medical School
Department
Miscellaneous
Type
Schools of Medicine
DUNS #
047006379
City
Boston
State
MA
Country
United States
Zip Code
02115