Genetic and physical methods will be used to determine the structures of the primary products of bacteriophage lambda recombination in order to define and distinguish the roles of bacterial and phage recombination functions. The function(s) responsible for adenine methylation independent mismatch will be characterized. The structures of lysogenic integration of Mu and the products of abortive Mu infection in him strains of E. coli will be characterized. An assay for in vivo inverted dimer formation arising from double strand breaks in the DNA of S. cerevisiae will be devised in order to identify and characterize the functions responsible for this fusion transaction.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37AI005388-29
Application #
3480384
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1977-12-01
Project End
1992-11-30
Budget Start
1990-12-01
Budget End
1991-11-30
Support Year
29
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Godoy, V G; Fox, M S (2000) Transposon stability and a role for conjugational transfer in adaptive mutability. Proc Natl Acad Sci U S A 97:7393-8
Godoy, V G; Gizatullin, F S; Fox, M S (2000) Some features of the mutability of bacteria during nonlethal selection. Genetics 154:49-59
Fox, M S; Radicella, J P; Yamamoto, K (1994) Some features of base pair mismatch repair and its role in the formation of genetic recombinants. Experientia 50:253-60
Radicella, J P; Clark, E A; Chen, S et al. (1993) Patch length of localized repair events: role of DNA polymerase I in mutY-dependent mismatch repair. J Bacteriol 175:7732-6
Kunes, S; Botstein, D; Fox, M S (1990) Synapsis-mediated fusion of free DNA ends forms inverted dimer plasmids in yeast. Genetics 124:67-80
Pearson, R K; Fox, M S (1988) Effects of DNA heterologies on bacteriophage lambda recombination. Genetics 118:13-9
Pearson, R K; Fox, M S (1988) Effects of DNA heterologies on bacteriophage lambda packaging. Genetics 118:5-12
Kunes, S; Ma, H; Overbye, K et al. (1987) Fine structure recombinational analysis of cloned genes using yeast transformation. Genetics 115:73-81