Abstract

Studies are proposed in four areas of cellular and molecular immunology. 1. Studies on a T helper stimulatory factor, THSF, a new lymphokine described by us in the previous grant period. THSF is derived from a cloned thymic epithelial cell line and appears to act on peripheral L3T4 T cells to coinduce the synthesis of lL2. (a) The factor will be purified to homogeneity and the cDNA coding for THSF will be identified and sequenced. (b) The purified factor will be used in receptor ligand studies. (c) A biological study will be carried out to determine the number of cell types upon which THSF acts and the precise nature of its effect. 2. Two lines of analysis are proposed to investigate the nature of T cell-B cell interactions, one, direct visualization studies with living stained cells, two, an analysis of T cell-B cell interactions in B cell differentiation. 3. Studies on the role antigen in the differentiation phase of the B cell response analysed with cells from transgenic mice expressing rearranged immunoglobulin genes with cells expressing the V-ese gene in the B cell response to High-SRBC. 4. The development of an experimental model to investigate the role of T cells and T cell products in the regulation of B cells isotypes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37AI008795-21
Application #
3480481
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1978-12-01
Project End
1992-11-30
Budget Start
1988-12-01
Budget End
1989-11-30
Support Year
21
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of California San Diego
Department
Type
Schools of Medicine
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093

  Publications

Thor, G; Sepulveda, H; Chada, S et al. (1993) Monoclonal antibody that distinguishes between a phosphorylated, beta 2-microglobulin-associated, and a free, nonphosphorylated, chain of MHC class I. J Immunol 151:211-24
Muralidhar, G; Sepulveda, H; Feeney, A J et al. (1992) Restricted IgH V gene usage in the response to the ese epitope on ""Hi"" sheep red blood cells. J Immunol 149:3574-9
Voland, J R; Wyzykowski, R J; Huang, M et al. (1992) Cloning and sequencing of a trophoblast-endothelial-activated lymphocyte surface protein: cDNA sequence and genomic structure. Proc Natl Acad Sci U S A 89:10425-9
Kubota, E; McKenzie, D T; Dutton, R W et al. (1991) Role of T cells in the B-cell response: glutaraldehyde-fixed T-helper hybridoma cells synergize with the lymphokine IL-4 to induce B-cell activation and proliferation. Immunology 72:40-7
Tonkonogy, S L; McKenzie, D T; Swain, S L (1989) Regulation of isotype production by IL-4 and IL-5. Effects of lymphokines on Ig production depend on the state of activation of the responding B cells. J Immunol 142:4351-60
Southern, S O; Dutton, R W (1989) A role for class I MHC antigens in responses of activated B lymphocytes. J Immunol 142:3384-91
Swain, S L; Dutton, R W; McKenzie, D et al. (1988) Role of antigen in the B cell response. Specific antigen and the lymphokine IL-5 synergize to drive B cell lymphoma proliferation and differentiation to Ig secretion. J Immunol 140:4224-30
Tonkonogy, S L; Swain, S L (1988) Stimulation of gut-associated lymphoid cells by IL-4 and B-cell growth factor II. Immunology 64:155-61
McKenzie, D (1988) Alloantigen presentation by B cells. Requirement for IL-1 and IL-6. J Immunol 141:2907-11
Swain, S L; Dutton, R W (1987) B cell growth factor interactions. Adv Exp Med Biol 213:215-25

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