Neutron scattering techniques will be used to elucidate the structure and function of the ribosome of E. coli. The following objectives will be pursued: (1) completion of a map of the 30S ribosomal subunit placing all 21 proteins in three dimensions, (2) direct measurement of the in situ radii of the 30S ribosomal proteins, (3) determination of the relative placement of the two subunits in the 70S couple, (4) determination of the position of tRNA when it is ribosome-bound, and (5) exploration of the possibility of mapping the location of segments of RNA within the ribosome. These projects all relate to the goal of understanding how the ribosome is constructed and how it works. The ribosome's function, the catalysis of protein synthesis, is a central one in all cells, in all organisms. The particle's RNA-based activity is likely to be both novel enzymologically as well biologically important.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37AI009167-26
Application #
2059208
Study Section
Special Emphasis Panel (NSS)
Project Start
1978-05-01
Project End
1995-12-14
Budget Start
1994-05-01
Budget End
1995-12-14
Support Year
26
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Yale University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520