The overall objective of this proposed research is to study the role of the Q and TL subregions of the murine histocompatibility complex in differentiation and development. These genetic regions encode for cell surface molecules with characteristics very similar to those involved in cell-cell communications during an immune response.
The specific aims for the next five years are as follows: (1) to continue our study of the ontogeny of the class I antigens determined by the Q and TL subregions; (2) to determine the influence of the Q and TL subregions on early embryonic development; (3) to determine the relationship between t-mutant genes and Q/TL genes; (4) to investigate the regulation of expression of the Qa/TL genes; and (5) to continue to production of genetic tools necessary for the continued investigation of the Q and TL subregions. Techniques to be used include cytotoxicity and ELISA techniques, Northern blot analyses, hematopoietic assays, transfection experiments, production of transgenic mice, analysis of the in vivo effects of monoclonal antibodies, genetic complementation tests, in vitro expression studies, and the production and analysis of new congenic strains of mice.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37AI012603-18
Application #
3480688
Study Section
Special Emphasis Panel (NSS)
Project Start
1991-05-01
Project End
1994-04-30
Budget Start
1992-05-01
Budget End
1993-04-30
Support Year
18
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Wadsworth Center
Department
Type
DUNS #
110521739
City
Menands
State
NY
Country
United States
Zip Code
12204
Herron, B J; Silva, G H; Flaherty, L (1998) Putative assignment of ESTs to the genetic map by use of the SSLP database. Mamm Genome 9:1072-4
Flaherty, L (1998) Generation, identification, and recovery of mouse mutations. Methods 14:107-18
Baldocchi, R A; Flaherty, L (1997) Isolation of genomic fragments from polymorphic regions by representational difference analysis. Methods 13:337-46
Wang, Q; Flaherty, L (1996) Developmental expression of the mouse MHC Q genes. Eur J Immunogenet 23:121-7
Wang, Q; Geliebter, J; Tonkonogy, S et al. (1993) Expression of the Q2 gene of the MHC in thymus and intestinal epithelial cells. Immunogenetics 38:370-2
Wade, N; Flaherty, L (1993) DNA sequence analysis and polymorphism of the mouse CD4 gene. Immunogenetics 38:384
Waters, J B; Flaherty, L (1991) Expression and regulation of Q8b in a transfected cell line. Immunogenetics 34:179-84
Walsh, A C; Slack, J; Flaherty, L (1991) Characterization of several new MHC congenic strains. Immunogenetics 33:290-3
Tine, J A; Walsh, A; Rathbun, D et al. (1990) Genetic polymorphisms of Q region genes from wild-derived mice: implications for Q region evolution. Immunogenetics 31:315-25
Widacki, S M; Mehta, V; Flaherty, L et al. (1990) Biochemical differences in Qa-2 antigens expressed by Qa-2+,6+ and Qa-2+,6- strains. Evidence for differential expression of the Q7 and Q9 genes. Mol Immunol 27:559-70

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