Panels of monoclonal antibodies and cDNA probes to lineage restricted and other genes are being used to thoroughly characterize B lymphocyte progenitors in the mouse. The objective is to establish a temporal sequence in which genes are expressed and/or repressed during differentiation and relate these to the rearrangement and usage of immunoglobulin genes. Soluble mediators, including a new pre-B cell growth factor, are being used to influence these events in short term cultures and long term cultures are being used to study microenvironmental elements which may normally elaborate such stimuli. Antibodies and gene probes are being used to characterize clones of stromal cells isolated from this model with a view to learning their diversity and developmental origins. Attempts will be made to understand how physical relationships between cells and directed migration are achieved within bone marrow and this will be related to immunohistochemical studies of marrow architecture. The basic information about the cellular and molecular requirements for B lymphocyte formation should contribute to our overall understanding of this differentiation sequence and may have practical implications relative to human disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37AI020069-09
Application #
3481092
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1982-09-01
Project End
1993-11-30
Budget Start
1990-12-01
Budget End
1991-11-30
Support Year
9
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Oklahoma Medical Research Foundation
Department
Type
DUNS #
937727907
City
Oklahoma City
State
OK
Country
United States
Zip Code
73104
Welner, Robert S; Kincade, Paul W (2014) 9-1-1: HSCs respond to emergency calls. Cell Stem Cell 14:415-6
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