T cells with Fc receptors for different immunoglobulin isotypes (IgM, IgG, IgA and IgE) have been described. Likewise, T cell-derived soluble factors with similar specificity for isotypes have been demonstrated. We postulate, the existence of similar T cells and factors specific for IgD; preliminary evidence supports this assumption. Furthermore, we hypothesize, that these FcdeltaR+ T cells and soluble IgD-binding factors produced by them, have multiple and widespread regulatory functions on the B cell system as a whole. Capitalizing on the experience of one of us (K. Ishizaka), in the identification of Fc-epsilon-R+ T cells and of soluble IgE-binding factors, we plan to isolate analogous IgD-binding factors. We plan to study the regulatory function of FcdeltaR+ T cells and soluble factors on the regulation of the IgM/IgD ratio on the membranes of B lymphocytes and on antibody production """"""""in vitro"""""""". We also plan to study the role of IgD-binding factors on proliferation and maturation of B cells stimulated """"""""in vitro"""""""" with anti-IgM antibodies. We shall also study the effect of the same factors on the isotype switch of B cells induced """"""""in vitro"""""""" by LPS. In the last two series of experiments, the effect of T cell-derived IgD-binding factors will be compared with that of different lymphokines, produced by T cells, that are known to affect the same processes of B cell proliferation and differentiation.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37AI022980-05
Application #
3481229
Study Section
Immunobiology Study Section (IMB)
Project Start
1985-12-01
Project End
1990-11-30
Budget Start
1989-12-01
Budget End
1990-11-30
Support Year
5
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Columbia University (N.Y.)
Department
Type
Schools of Medicine
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10027
Guarini, L; Weber, D A; Pernis, B (1989) Differential endocytosis of IgM and IgD in murine B cell lines. Cell Immunol 123:456-61
Adachi, M; Ishizaka, K (1988) Enhancement of antibody responses by IgD-binding factors induced by anti-IgD treatment of spleen cells. Proc Natl Acad Sci U S A 85:554-8
Adachi, M; Arai, N; Ishizaka, K (1988) Participation of IL-4 in the formation of IgD-binding factors by antigen-primed mouse spleen cells. J Immunol 141:2358-66
Adachi, M; Ishizaka, K (1986) IgD-binding factors from mouse T lymphocytes. Proc Natl Acad Sci U S A 83:7003-7