Abstract

specific for sulfhydryl groups and N-hydroxy-chloroacetylaminoarene substrates to identify amino acids that are crucial to the catalytic activity. the monoclonal antibodies will be used to help in establishing the areas of the molecule that are involved in catalysis. (3. Localize the acetyltransferase and mRNA in rat tissues by use of monoclonal antibodies and cDNA probes to identify those cells that may be at particular risk from these compounds. (4. Express acetyltransferase in bacterial and mammalian cells to verify the identity of the sequence and to provide a means by which cells can be provided with a metabolic activation system. (5. Compare the structures of acetyltransferases from other species that differ in their abilities to carry out N-,O- and N,O- acetyltransfer.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
2R37CA023386-12
Application #
3481961
Study Section
Chemical Pathology Study Section (CPA)
Project Start
1978-04-01
Project End
1994-03-31
Budget Start
1989-04-01
Budget End
1990-03-31
Support Year
12
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Barbara Ann Karmanos Cancer Institute
Department
Type
DUNS #
City
Detroit
State
MI
Country
United States
Zip Code
48201

  Publications

Soos, Gyorgyike; Haas, Gabriel P; Wang, Ching Y et al. (2003) Differential gene expression in human prostate cancer cells adapted to growth in bone in Beige mice. Urol Oncol 21:15-9
Wang, Ching Y; Jones, Richard F; Debiec-Rychter, Maria et al. (2002) Correlation of the genotypes for N-acetyltransferases 1 and 2 with double bladder and prostate cancers in a case-comparison study. Anticancer Res 22:3529-35
Archer, C L; Morse, P; Jones, R F et al. (2000) Carcinogenicity of the N-hydroxy derivative of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, 2-amino-3, 8-dimethyl-imidazo[4,5-f]quinoxaline and 3, 2'-dimethyl-4-aminobiphenyl in the rat. Cancer Lett 155:55-60
Singh, A; Jones, R F; Friedman, H et al. (1999) Expression of p53 and pRb in bladder and prostate cancers of patients having both cancers. Anticancer Res 19:5415-7
Archer, C; Debiec-Rychter, M; Morse, P et al. (1999) Epithelial proliferation and expression of the aromatic amine activation enzyme N-acetyltransferase in the prostate of postnatal rat. Anticancer Res 19:4013-6
Vosianov, A F; Romanenko, A M; Zabarko, L B et al. (1999) Prostatic intraepithelial neoplasia and apoptosis in benign prostatic hyperplasia before and after the Chernobyl accident in Ukraine. Pathol Oncol Res 5:28-31
Wang, C Y; Debiec-Rychter, M; Schut, H A et al. (1999) N-Acetyltransferase expression and DNA binding of N-hydroxyheterocyclic amines in human prostate epithelium. Carcinogenesis 20:1591-5
Debiec-Rychter, M; Tada, M; Poirier, M C et al. (1998) DNA adduct formation in human and rat mammary epithelium by N-hydroxy derivatives of 2-aminofluorene and 4-aminobiphenyl. Teratog Carcinog Mutagen 18:35-9
Soos, G; Jones, R F; Haas, G P et al. (1997) Comparative intraosseal growth of human prostate cancer cell lines LNCaP and PC-3 in the nude mouse. Anticancer Res 17:4253-8
Hirose, M; Shirai, T; Lee, M S et al. (1997) Carcinogenecity of the N-acyl derivatives of N-hydroxy-trans-4-aminostilbene in CD rats. Chem Biol Interact 106:123-32

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