A study of radiation effects is proposed which will employ electron spin resonance (ESR) spectroscopy and electron-nuclear double resonance (ENDOR) spectroscopy to identify direct radiation damage in irradiated single crystals of biomolecules. A principal objective will be to synthesize and crystallize DNA oligomers of sufficient length for meaningful ESR-ENDOR studies of radiation damage in nucleic acids. Paramagnetic one-electron oxidation and reduction products are observed in this type of study. An objective of the study is to relate these primary species to the final products of direct radiation damage which form in subsequent reactions of the primary radical species. Another objective is the characterization of electrons trapped in defined positions in single crystals. V band spectroscopy has been developed to the point where not only nearby protons associated with the polar tapping groups but also distant protons can be detected by ENDOR. This development makes detailed characterization of electrons trapped in a polar environment possible.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37CA025027-27
Application #
2087313
Study Section
Special Emphasis Panel (NSS)
Project Start
1978-08-01
Project End
1995-11-30
Budget Start
1993-12-01
Budget End
1995-11-30
Support Year
27
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Roswell Park Cancer Institute Corp
Department
Type
DUNS #
City
Buffalo
State
NY
Country
United States
Zip Code
14263
Box, H C; Budzinski, E E; Dawidzik, J D et al. (1996) Radiation-induced formation of a crosslink between base moieties of deoxyguanosine and thymidine in deoxygenated solutions of d(CpGpTpA). Radiat Res 145:641-3
Schroder, E; Budzinski, E E; Wallace, J C et al. (1995) Radiation chemistry of d(ApCpGpT). Int J Radiat Biol 68:509-23
Box, H C; Freund, H G; Budzinski, E E et al. (1995) Free radical-induced double base lesions. Radiat Res 141:91-4
Maccubbin, A E; Przybyszewski, J; Evans, M S et al. (1995) DNA damage in UVB-irradiated keratinocytes. Carcinogenesis 16:1659-60
Maccubbin, A E; Budzinski, E E; Patrzyc, H et al. (1993) 32P-postlabeling assay for free radical-induced DNA damage: the formamido remnant of thymine. Free Radic Res Commun 18:17-28
Budzinski, E E; Maccubbin, A E; Freund, H G et al. (1993) Characterization of the products of dinucleoside monophosphates d(GpN) irradiated in aqueous solutions. Radiat Res 136:171-7
Box, H C; Freund, H G; Budzinski, E E (1990) An unusual electron trapping site in the crystal structure of dulcitol. Radiat Res 122:137-41
Box, H C; Budzinski, E E; Freund, H G (1990) Studies of electrons trapped in X-irradiated rhamnose crystals. Radiat Res 121:262-6
Paul, C R; Wallace, J C; Alderfer, J L et al. (1988) Radiation chemistry of d(TpApCpG) in oxygenated solution. Int J Radiat Biol 54:403-15
Paul, C R; Arakali, A V; Box, H C (1988) Radioprotection of irradiated DNA and its model compounds. Pharmacol Ther 39:149-50

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