Mason-Pfizer Monkey virus (M-PMV) is an exogenous, primate retrovirus that was isolated from a breast carcinoma of a rhesus monkey. It is the prototype virus of the D-type retroviruses which, like the B type retrovirus mouse mammary tumor virus, preassemble a complete capsid within the cytoplasm of a infected cell. These A-type particles migrate to the plasma membrane and are enveloped by a lipid bilayer upon release from the cell. The major goals of this proposal are to define those viral coded gag-gene functions that are essential in assembly, morphogenesis and infectivity of the M-PMV particle. The carboxy-terminal amino acid sequence of the five major gag gene products of this virus will be determined. This information will be used together with amino-terminal amino acid sequence information to define the polypeptide coding regions on the nucleic acid sequence of the gag gene determined from cloned M-PMV DNA. In vitro site directed mutagenesis of the gag gene coding region and of a sequence 3' of gag which may code for the viral protease will be used to determine the function of these regions. Deletion, insertion, and point mutagenesis procedures will be employed for these studies. The role of the envelope glycoproteins in directing the budding and release of viral particales will be investigated utilizing similar procedures.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37CA027834-12
Application #
3482085
Study Section
Experimental Virology Study Section (EVR)
Project Start
1980-09-01
Project End
1996-02-28
Budget Start
1992-02-29
Budget End
1993-02-28
Support Year
12
Fiscal Year
1992
Total Cost
Indirect Cost
Name
University of Alabama Birmingham
Department
Type
Schools of Dentistry
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294
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Wen, Xiaoyun; Ding, Lingmei; Hunter, Eric et al. (2014) An siRNA screen of membrane trafficking genes highlights pathways common to HIV-1 and M-PMV virus assembly and release. PLoS One 9:e106151
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Bohmova, Karolina; Hadravova, Romana; Stokrova, Jitka et al. (2010) Effect of dimerizing domains and basic residues on in vitro and in vivo assembly of Mason-Pfizer monkey virus and human immunodeficiency virus. J Virol 84:1977-88
Knejzlik, Zdenýýk; Ulbrich, Pavel; Strohalm, Martin et al. (2009) Conformational changes of the N-terminal part of Mason-Pfizer monkey virus p12 protein during multimerization. Virology 393:168-76

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