Abstract

DNA probes for individual gene products of both the endocrine pancreas (insulin and potentially glucagon) and the exocrine pancreas (several digestive enzymes including amylase) have been prepared by reverse transcription of mRNA and subsequent cloning in E. coli X1776. We propose to utilize the cloned structural gene sequences to probe the organization and expression of the endocrine and exocrine specific genes. The cloned sequences will be used to isolate (by cloning in E. coli X1776) insulin and amylase gene sequences from rat nuclear DNA. The isolaed genes will permit the study of the sequence organization of the structural genes and their contiguous DNA regions. The cloned structural genes will also be used to study changes in their expression during embryonic development as well as alteration in expression accompanying pathogenic states.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37DK021344-12
Application #
3483379
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1980-12-01
Project End
1992-08-31
Budget Start
1988-09-01
Budget End
1989-08-31
Support Year
12
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Shimajiri, Yoshinori; Kosaka, Yasuhiro; Scheel, David W et al. (2011) A mouse model for monitoring islet cell genesis and developing therapies for diabetes. Dis Model Mech 4:268-76
Gasa, Rosa; Mrejen, Caroline; Lynn, Francis C et al. (2008) Induction of pancreatic islet cell differentiation by the neurogenin-neuroD cascade. Differentiation 76:381-91
Lynn, F C; Smith, S B; Wilson, M E et al. (2007) Sox9 coordinates a transcriptional network in pancreatic progenitor cells. Proc Natl Acad Sci U S A 104:10500-5
Smith, Stuart B; Watada, Hirotaka; German, Michael S (2004) Neurogenin3 activates the islet differentiation program while repressing its own expression. Mol Endocrinol 18:142-9
Smith, Stuart B; Gasa, Rosa; Watada, Hirotaka et al. (2003) Neurogenin3 and hepatic nuclear factor 1 cooperate in activating pancreatic expression of Pax4. J Biol Chem 278:38254-9
Watada, Hirotaka; Scheel, David W; Leung, Joey et al. (2003) Distinct gene expression programs function in progenitor and mature islet cells. J Biol Chem 278:17130-40
Wilson, Maria E; Scheel, David; German, Michael S (2003) Gene expression cascades in pancreatic development. Mech Dev 120:65-80
Thameem, Farook; Wolford, Johanna K; Wang, Juehu et al. (2002) Cloning, expression and genomic structure of human LMX1A, and variant screening in Pima Indians. Gene 290:217-25
Lee, J C; Smith, S B; Watada, H et al. (2001) Regulation of the pancreatic pro-endocrine gene neurogenin3. Diabetes 50:928-36
Ohneda, K; Mirmira, R G; Wang, J et al. (2000) The homeodomain of PDX-1 mediates multiple protein-protein interactions in the formation of a transcriptional activation complex on the insulin promoter. Mol Cell Biol 20:900-11

Showing the most recent 10 out of 42 publications