Abstract

Basement membranes (BMs) are cell-associated extracellular matrices formed initially as laminin Dolymer scaffoldings. They act as solid-phase agonists for adherent tissue cells, profoundly affecting their differentiation, organization and maintenance. Defects of BM components and structure are a cause of Aim I. Role of laminin LG domain-dependent anchorage.
Aim II. Post-anchorage laminin and basement membrane functions.
Aim 1 11. Architectural and other laminin short-arm functions. To address these, we propose to integrate the approaches of using recombinant laminins with specific function- altering mutations to analyze assembly and cell differentiation in normal and genetically-modified EBs, renal tubular epithelial and other BM-assembling cells backed by mouse genetics to validate our hypotheses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37DK036425-25
Application #
7744020
Study Section
Special Emphasis Panel (NSS)
Program Officer
Ketchum, Christian J
Project Start
1986-01-01
Project End
2012-12-31
Budget Start
2010-01-01
Budget End
2010-12-31
Support Year
25
Fiscal Year
2010
Total Cost
$275,301
Indirect Cost

  Institution

Name
University of Medicine & Dentistry of NJ
Department
Pathology
Type
Schools of Medicine
DUNS #
617022384
City
Piscataway
State
NJ
Country
United States
Zip Code
08854

  Publications

van Wijk, Xander M; Döhrmann, Simon; Hallström, Björn M et al. (2017) Whole-Genome Sequencing of Invasion-Resistant Cells Identifies Laminin ?2 as a Host Factor for Bacterial Invasion. MBio 8:
Pozzi, Ambra; Yurchenco, Peter D; Iozzo, Renato V (2017) The nature and biology of basement membranes. Matrix Biol 57-58:1-11
Colombelli, Cristina; Palmisano, Marilena; Eshed-Eisenbach, Yael et al. (2015) Perlecan is recruited by dystroglycan to nodes of Ranvier and binds the clustering molecule gliomedin. J Cell Biol 208:313-29
Hohenester, Erhard; Yurchenco, Peter D (2013) Laminins in basement membrane assembly. Cell Adh Migr 7:56-63
McKee, Karen K; Yang, Dong-Hua; Patel, Rajesh et al. (2012) Schwann cell myelination requires integration of laminin activities. J Cell Sci 125:4609-19
Yurchenco, Peter D (2011) Basement membranes: cell scaffoldings and signaling platforms. Cold Spring Harb Perspect Biol 3:
Yang, Dong-Hua; McKee, Karen K; Chen, Zu-Lin et al. (2011) Renal collecting system growth and function depend upon embryonic ?1 laminin expression. Development 138:4535-44
He, Xiaowen; Liu, Jie; Qi, Yanmei et al. (2010) Rac1 is essential for basement membrane-dependent epiblast survival. Mol Cell Biol 30:3569-81
McKee, Karen K; Capizzi, Stephanie; Yurchenco, Peter D (2009) Scaffold-forming and Adhesive Contributions of Synthetic Laminin-binding Proteins to Basement Membrane Assembly. J Biol Chem 284:8984-94
Yurchenco, Peter D; Patton, Bruce L (2009) Developmental and pathogenic mechanisms of basement membrane assembly. Curr Pharm Des 15:1277-94

Showing the most recent 10 out of 35 publications