1. Tissue culture media will be modified in an attempt to cause tissue cultured corneal endothelial monolayers to acquire their normal functional state as indicated by their electrical properties. 2. Perfusion media will be modified so as to maintain the cornea of a perfused isolated eye in good condition for as long as possible. The behavior of the tissue will be compared with that of an isolated cornea perfused with the same medium, in order to test whether a component secreted by the ciliary body is essential to its welfare. 3. The kinetics of the different cell types within an injured corneal stroma will be followed in order to clarify their origins, transformations and fate. 4. The cellular reactions to corneal injuries in the isolated perfused eye, in the confines of the stroma, and under a glued-on on contact lens will be examined, with a view to identifying the cellular and stimulatory contributions from the blood and tears. 5. Liposomes suspended in physiological medium will be used as indicators of fluid flow across the endothelial surface in order to determine whether the fluid pump operates between or across the cells. 6. An electrochemical potential difference of Na, K, Cl and HCO3 across the endothelium will be looked for by bringing ion- sensitive electrodes close to the cell layer and blocking ion fluxes. The development of a potential step will establish that an ion is involved in an active transport mechanism.

National Institute of Health (NIH)
National Eye Institute (NEI)
Method to Extend Research in Time (MERIT) Award (R37)
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Special Emphasis Panel (NSS)
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Columbia University (N.Y.)
Schools of Medicine
New York
United States
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Nagasaki, Takayuki; Zhao, Jin (2005) Uniform distribution of epithelial stem cells in the bulbar conjunctiva. Invest Ophthalmol Vis Sci 46:126-32
Smith, R T; Chan, J K; Nagasaki, T et al. (2005) A method of drusen measurement based on reconstruction of fundus background reflectance. Br J Ophthalmol 89:87-91
Fischbarg, Jorge; Maurice, David M (2004) An update on corneal hydration control. Exp Eye Res 78:537-41
Maurice, D M; Zhao, J; Nagasaki, T (2004) A novel microscope system for time-lapse observation of corneal cells in a living mouse. Exp Eye Res 78:591-7
Zhao, Jin; Nagasaki, Takayuki (2004) Mechanical damage to corneal stromal cells by epithelial scraping. Cornea 23:497-502
Zhao, Jin; Nagasaki, Takayuki (2003) Lacrimal gland as the major source of mouse tear factors that are cytotoxic to corneal keratocytes. Exp Eye Res 77:297-304
Nagasaki, Takayuki; Zhao, Jin (2003) Centripetal movement of corneal epithelial cells in the normal adult mouse. Invest Ophthalmol Vis Sci 44:558-66
Maurice, David M (2002) Drug delivery to the posterior segment from drops. Surv Ophthalmol 47 Suppl 1:S41-52
Maurice, D (2001) Review: practical issues in intravitreal drug delivery. J Ocul Pharmacol Ther 17:393-401
Zhao, J; Nagasaki, T; Maurice, D M (2001) Role of tears in keratocyte loss after epithelial removal in mouse cornea. Invest Ophthalmol Vis Sci 42:1743-9

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