NMR Spectroscopy will continue to be applied to determine the structure and dynamics of transfer RNA in interaction with the enzyme aminoacyl tRNA synthetase. These two macromolecules are essential parts of the biosynthetic machinery of all living things. The emphasis is on use of isotope labels of 15N and 13C to highlight proton NMR signals and provide more information than straight NMR spectroscopy. Smaller molecules interacting with the enzyme, as well as labelled enzyme and labelled tRNA, will be examined to provide information on molecular changes during function of the molecules. The NMR methods being used are prototypical of methods potentially applicable to drug design.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37GM020168-23
Application #
2173547
Study Section
Special Emphasis Panel (NSS)
Project Start
1976-06-01
Project End
1996-07-31
Budget Start
1995-08-01
Budget End
1996-07-31
Support Year
23
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Brandeis University
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
616845814
City
Waltham
State
MA
Country
United States
Zip Code
02454
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Hall, K B; McLaughlin, L W (1991) Thermodynamic and structural properties of pentamer DNA.DNA, RNA.RNA, and DNA.RNA duplexes of identical sequence. Biochemistry 30:10606-13

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