The long term goal of this research is to understand the function and regulation of the heat shock response in eukaryotes, using budding yeast as a model system. Yeast has two classes of abundant but functionally distinct heat shock 70K proteins (hsp70s)-four SSA and two SSB proteins-and several proteins related to E. coli DnaJ. These proteins function in vivo as molecular chaperones in the cellular processes of protein maturation and regulation of the heat shock response. Using genetic, molecular, and biochemical approaches the investigator will analyze the function of the two groups of cytosolic hsp70 proteins in translation, protein folding, proteolysis, and regulation of the heat shock response. There are five specific aims. The first is to examine the function of the SSB proteins in protein synthesis in vivo. SSB proteins are associated with translating ribosomes and are required for efficient translation. Genetic, molecular, and biochemical approaches will be used in an attempt to get at the mechanism of action of SSB proteins in the translation process. These include: analysis of SSA/SSB chimeras to define regions critical for the specific functions of the SSB proteins; analysis of high copy and extragenic suppressors of the cold- sensitive phenotype of ssb1ssb2 mutants; identification of proteins that interact with Ssbs; determination of the stoichiometry of Ssbs with respect to ribosomes; definition of the nature of the interaction of Ssbs with translating ribosomes; and the mechanism of Ssb action in translation. The second specific aim is to examine the function of SSA proteins in protein folding and proteolysis in vivo. The role of these proteins in protein maturation will be studied by examining the effects of ssa mutations on the folding of several test proteins. The role of SSA proteins in proteolysis will be studied by measuring the effect of mutations in the SSA genes on the degradation of specific substrates of the ubiquitin based proteolysis system. The genetic interactions between ssa mutants and ubi mutants will be examined to look for additional links between the chaperones and proteolysis. The third specific aim is to define the differences between the SSA and SSB proteins. The SSA and SSB proteins will be purified and characterized biochemically for such parameters as peptide-stimulated ATPase activity and peptide binding. The fourth specific aim is to determine whether the DnaJ-like proteins of yeast (Sis1 and Ydj1) function in pairs with specific Hsp70s. Genetic and phenotypic comparisons will be made between mutants in the SSA, SSB, and dnaJ-like genes. Biochemical assays will be performed with purified chaperones to assess the roles of the DnaJ proteins in several functions of the SSA and SSB proteins. These include stimulation of the ATPase activity of the SSA protein and the refolding of test polypeptide substrates by both Hsp70s. The final specific aim is to examine the regulation of gene expression upon heat shock. The role of SSA proteins in the regulation of HSP gene expression will be characterized with appropriate mutations in SSA genes, and the involvement of HSF-phosphorylation in this response will be determined. The mechanism responsible for the decrease in SSB gene expression upon heat shock will also be determined.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Method to Extend Research in Time (MERIT) Award (R37)
Project #
Application #
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Program Officer
Anderson, James J
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Wisconsin Madison
Schools of Medicine
United States
Zip Code
Lee, Kanghyun; Sharma, Ruchika; Shrestha, Om Kumar et al. (2016) Dual interaction of the Hsp70 J-protein cochaperone Zuotin with the 40S and 60S ribosomal subunits. Nat Struct Mol Biol 23:1003-1010
Yonashiro, Ryo; Tahara, Erich B; Bengtson, Mario H et al. (2016) The Rqc2/Tae2 subunit of the ribosome-associated quality control (RQC) complex marks ribosome-stalled nascent polypeptide chains for aggregation. Elife 5:e11794
Kaschner, Lindsey A; Sharma, Ruchika; Shrestha, Om Kumar et al. (2015) A conserved domain important for association of eukaryotic J-protein co-chaperones Jjj1 and Zuo1 with the ribosome. Biochim Biophys Acta 1853:1035-45
Yu, Hyun Young; Ziegelhoffer, Thomas; Craig, Elizabeth A (2015) Functionality of Class A and Class B J-protein co-chaperones with Hsp70. FEBS Lett 589:2825-30
Yu, Hyun Young; Ziegelhoffer, Thomas; Osipiuk, Jerzy et al. (2015) Roles of intramolecular and intermolecular interactions in functional regulation of the Hsp70 J-protein co-chaperone Sis1. J Mol Biol 427:1632-43
Ciesielski, Grzegorz L; Plotka, Magdalena; Manicki, Mateusz et al. (2013) Nucleoid localization of Hsp40 Mdj1 is important for its function in maintenance of mitochondrial DNA. Biochim Biophys Acta 1833:2233-43
Sahi, Chandan; Kominek, Jacek; Ziegelhoffer, Thomas et al. (2013) Sequential duplications of an ancient member of the DnaJ-family expanded the functional chaperone network in the eukaryotic cytosol. Mol Biol Evol 30:985-98
Kominek, Jacek; Marszalek, Jaroslaw; Neuvéglise, Cécile et al. (2013) The complex evolutionary dynamics of Hsp70s: a genomic and functional perspective. Genome Biol Evol 5:2460-77
Ducett, Jeanette K; Peterson, Francis C; Hoover, Lindsey A et al. (2013) Unfolding of the C-terminal domain of the J-protein Zuo1 releases autoinhibition and activates Pdr1-dependent transcription. J Mol Biol 425:19-31
Prunuske, Amy J; Waltner, Jeanette K; Kuhn, Peter et al. (2012) Role for the molecular chaperones Zuo1 and Ssz1 in quorum sensing via activation of the transcription factor Pdr1. Proc Natl Acad Sci U S A 109:472-7

Showing the most recent 10 out of 28 publications