Light signals from the environment are perceived by distinct classes of photoreceptors and transduced to the nuclear regulators to control the developmental modes, largely through regulated gene expression. The long-term goal of our research is to dissect the cellular and biochemical basis of this light control of development switch. Our previous work, together with studies from others, has defined a group of evolutionarily conserved COP/DET/FUS proteins that act as light inactivatable repressor of photomorphogenesis. We have established that Arabidopsis COP1 (constitutively photomorphogenic 1) acts within nucleus to suppress the photomorphogenic developmental pathway in darkness and light reverses this repressive action and deplete its nuclear accumulation. Our current NIH funded studies indicates that once within the nucleus, COP1, together with COP10 complex, the COP9 signalosome, and DET1 complex, physically contact and target specific transcription factors (including HY5 and HYH) for ubiquitination and proteasome mediated degradation. The activities of those transcription factors are responsible for promoting photomorphogenic development and gene expression. We also made important progresses in understanding how blue and far red light, once perceived by cryptochromes and phytochrome A, transduced to regulate this switch. Here I propose to combine molecular genetic, biochemical, proteomic, and genomic approaches to comprehensively analyze this regulatory network at the levels of biochemical composition and action, network interactions of the components, and their mediation of light control of genome expression and development. To achieve this goal, I propose three major complementary aspects of further research with eight specific Objectives. The first aspect, including Objectives I to III, is basically to confirm the hypothesized biochemical activities and to reveal the molecular constitutes of the two major players, the COP1 and COP10 complexes. The second aspect, including Objectives IV and VI, is to how far red and blue light signal regulate the COP1 switch through their own photoreceptors and signaling events. The third aspect, including Objectives VII to VIII, is to provide a whole genome level understanding on light control of gene expression and reveal the transcriptional cascade/network at a genomic scale. The accomplishment of these proposed objectives shall provide a comprehensive and mechanistic view of the signaling network responsible for light control of development. This should serve as an essential guidance for investigating similar issues in mammals including human. ? ?

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Method to Extend Research in Time (MERIT) Award (R37)
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Cell Development and Function Integrated Review Group (CDF)
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Anderson, James J
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Yale University
Schools of Arts and Sciences
New Haven
United States
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Zhou, Yangyang; Yang, Li; Duan, Jie et al. (2018) Hinge region of Arabidopsis phyA plays an important role in regulating phyA function. Proc Natl Acad Sci U S A 115:E11864-E11873
Zhao, Xianhai; Jiang, Yan; Li, Jian et al. (2018) COP1 SUPPRESSOR 4 promotes seedling photomorphogenesis by repressing CCA1 and PIF4 expression in Arabidopsis. Proc Natl Acad Sci U S A 115:11631-11636
Shi, Hui; Lyu, Mohan; Luo, Yiwen et al. (2018) Genome-wide regulation of light-controlled seedling morphogenesis by three families of transcription factors. Proc Natl Acad Sci U S A 115:6482-6487
Lin, Fang; Jiang, Yan; Li, Jian et al. (2018) B-BOX DOMAIN PROTEIN28 Negatively Regulates Photomorphogenesis by Repressing the Activity of Transcription Factor HY5 and Undergoes COP1-Mediated Degradation. Plant Cell 30:2006-2019
Lin, Fang; Xu, Dongqing; Jiang, Yan et al. (2017) Phosphorylation and negative regulation of CONSTITUTIVELY PHOTOMORPHOGENIC 1 by PINOID in Arabidopsis. Proc Natl Acad Sci U S A 114:6617-6622
Ling, Jun-Jie; Li, Jian; Zhu, Danmeng et al. (2017) Noncanonical role of Arabidopsis COP1/SPA complex in repressing BIN2-mediated PIF3 phosphorylation and degradation in darkness. Proc Natl Acad Sci U S A 114:3539-3544
Shi, Hui; Liu, Renlu; Xue, Chang et al. (2016) Seedlings Transduce the Depth and Mechanical Pressure of Covering Soil Using COP1 and Ethylene to Regulate EBF1/EBF2 for Soil Emergence. Curr Biol 26:139-149
Shi, Hui; Shen, Xing; Liu, Renlu et al. (2016) The Red Light Receptor Phytochrome B Directly Enhances Substrate-E3 Ligase Interactions to Attenuate Ethylene Responses. Dev Cell 39:597-610
Li, Kunlun; Yu, Renbo; Fan, Liu-Min et al. (2016) DELLA-mediated PIF degradation contributes to coordination of light and gibberellin signalling in Arabidopsis. Nat Commun 7:11868
Xu, Dongqing; Jiang, Yan; Li, Jigang et al. (2016) BBX21, an Arabidopsis B-box protein, directly activates HY5 and is targeted by COP1 for 26S proteasome-mediated degradation. Proc Natl Acad Sci U S A 113:7655-60

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