See attached continuation.
Split inteins are Nature's protein ligases, mediating the traceless ligation of any polypeptide chains to which they are appended. The research program focuses on a recently discovered family of split inteins that mediate this ligation process with unprecedented speed and fidelity, creating enormous potential in protein biotechnology with applications ranging from basic molecular and cell biology to the preparation of protein therapeutics. We will determine how these proteins catalyst the ligation reaction so efficiently and then use this information to develop therapeutic strategies targeting inteins and develop new technologies for manipulating the structure and function of proteins in vivo.
| Gramespacher, Josef A; Stevens, Adam J; Thompson, Robert E et al. (2018) Improved protein splicing using embedded split inteins. Protein Sci 27:614-619 |
| Jenness, Christopher; Giunta, Simona; Müller, Manuel M et al. (2018) HELLS and CDCA7 comprise a bipartite nucleosome remodeling complex defective in ICF syndrome. Proc Natl Acad Sci U S A 115:E876-E885 |
| Wojcik, Felix; Dann, Geoffrey P; Beh, Leslie Y et al. (2018) Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants. Nat Commun 9:1394 |
| David, Yael; Muir, Tom W (2017) Emerging Chemistry Strategies for Engineering Native Chromatin. J Am Chem Soc 139:9090-9096 |
| Gramespacher, Josef A; Stevens, Adam J; Nguyen, Duy P et al. (2017) Intein Zymogens: Conditional Assembly and Splicing of Split Inteins via Targeted Proteolysis. J Am Chem Soc 139:8074-8077 |
| Winer, Benjamin Y; Huang, Tiffany S; Pludwinski, Eitan et al. (2017) Long-term hepatitis B infection in a scalable hepatic co-culture system. Nat Commun 8:125 |
| Wang, Xueyin; Paucek, Richard D; Gooding, Anne R et al. (2017) Molecular analysis of PRC2 recruitment to DNA in chromatin and its inhibition by RNA. Nat Struct Mol Biol 24:1028-1038 |
| Dann, Geoffrey P; Liszczak, Glen P; Bagert, John D et al. (2017) ISWI chromatin remodellers sense nucleosome modifications to determine substrate preference. Nature 548:607-611 |
| Stevens, Adam J; Sekar, Giridhar; Shah, Neel H et al. (2017) A promiscuous split intein with expanded protein engineering applications. Proc Natl Acad Sci U S A 114:8538-8543 |
| Debelouchina, Galia T; Muir, Tom W (2017) A molecular engineering toolbox for the structural biologist. Q Rev Biophys 50:e7 |
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