This proposed research program is a direct extension of our earlier program with the same 1 fundamental goals: the investigation of reproduction, and the molecular mechanisms that control genetic activity.
Specific aims i nclude: determination of the biological significance of the individual cis regulatory sites of Cyllla and SM50 genes; cloning, characterization, and studies on the localization and synthesis of the trans regulatory factor directing expression of these genes; exploration of upper levels of the regulatory control hierarchy, including search for elements shared amongst coordinately controlled genes; experiments on normal and chimeric embryos using molecular probes to investigate induction and localization in early development; extension of knowledge of specification and cell lineage in the embryo, and determination of the origin of lineage elements using imaginal rudiments; carrying out studies on transcription in oogenesis: utilizing evolutionary change and conservation in sequences important for developmental gene regulation for assessment of functional significance; development of paradigms for photoperiodic control of gonadal cycle in cultured sea urchins producing and characterizing inbred lines of laboratory sea urchins, and attempting to-induce and exploit hermaphroditism for research purposes.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37HD005753-29
Application #
2673367
Study Section
Special Emphasis Panel (NSS)
Program Officer
Tasca, Richard J
Project Start
1974-05-01
Project End
2000-03-31
Budget Start
1998-04-01
Budget End
2000-03-31
Support Year
29
Fiscal Year
1998
Total Cost
Indirect Cost
Name
California Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
078731668
City
Pasadena
State
CA
Country
United States
Zip Code
91125
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