The long term goal of this application is to elucidate the in vivo significance and molecular mechanisms of integrin-mediated activation of transforming growth factor beta (TGFbeta). In current and previous cycles of this grant we have shown that the integrin, alphavbetaS, activates TGF beta and that this process plays a central role in models of pulmonary fibrosis and acute lung injury in mice. We have also identified three stimuli, thrombin, LPA and S1P that can each activate this process be initiating signals from G protein coupled receptors. In this application we propose to first determine the importance of this pathway in a more clinically relevant model of acute lung injury that combines low dose LPS with normal tidal volume ventilation. For these experiments we will also determine whether any protection seen with loss or blockade of the integrin is specifically due to loss of the integrin from alveolar epithelium and/or to loss of TGF beta activation using mice we have generated expressing either the integrin or active TGF beta as alveolar epithelial transgenes. In parallel, we will explore the mechanisms underlying indcution of this process through activation of G protein coupled receptors. We will use cells from mice lacking Galpha12, Galpha13 or Galphai and shRNA designed to knockdown each subunit to identify the G proteins involved in these responses. We will take a similar approach to identify the relevant LPA and S1P receptors. To genetically determine the relevance of actin-myosin mediated contraction, and to identify the specific myosin isoforms invovled, we will also use shRNA-mediated knockdown of each of the three myosin II isoforms we have identified in lung epithelial cells. To determine the significance of cell contraction we will also quantify alphavbeta6-medated TGF beta activation by cells plated on substrates of varying stiffness. Finally, we will use labeled forms of the integrin and a TGF beta receptor to perform live videomicroscopy and test the idea that this process involves the formation of a TGF beta activation synapse across adjacent cells. Lay summary: This application seeks to understand a key process that controls pulmonary fibrosis and acute lung injury, two common and currently largely untreatable diseases. By determining the mechansims by which cells in the lung control this process, we should be able to identify and validate novel targets for design of effective treatments for these diseases.
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| Li, John T; Melton, Andrew C; Su, George et al. (2015) Unexpected Role for Adaptive ??Th17 Cells in Acute Respiratory Distress Syndrome. J Immunol 195:87-95 |
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| Xu, Pinglong; Bailey-Bucktrout, Samantha; Xi, Ying et al. (2014) Innate antiviral host defense attenuates TGF-? function through IRF3-mediated suppression of Smad signaling. Mol Cell 56:723-37 |
| Madala, Satish K; Korfhagen, Thomas R; Schmidt, Stephanie et al. (2014) Inhibition of the ?v?6 integrin leads to limited alteration of TGF-?-induced pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol 306:L726-35 |
| Erle, David J; Sheppard, Dean (2014) The cell biology of asthma. J Cell Biol 205:621-31 |
| Arnold, Thomas D; Niaudet, Colin; Pang, Mei-Fong et al. (2014) Excessive vascular sprouting underlies cerebral hemorrhage in mice lacking ?V?8-TGF? signaling in the brain. Development 141:4489-99 |
| Henderson, Neil C; Sheppard, Dean (2013) Integrin-mediated regulation of TGF? in fibrosis. Biochim Biophys Acta 1832:891-6 |
| Su, George; Atakilit, Amha; Li, John T et al. (2013) Effective treatment of mouse sepsis with an inhibitory antibody targeting integrin *v*5. Crit Care Med 41:546-53 |
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